WB (Western Blot)
(Detection of human SPIN90 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody AAA211951 (lot AAA211951-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SPIN90 Polyclonal Antibody | anti-SPIN90 antibody
Rabbit anti-SPIN90 Antibody, Affinity Purified
Gene Names
NCKIPSD; DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SPIN90, Antibody; Rabbit anti-SPIN90 Antibody, Affinity Purified; WISH; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; SPIN90; VIP54; WASP-interacting SH3-domain protein; wiskott-Aldrich syndrome protein-interacting protein; dia-interacting protein 1; NCK-interacting protein with SH3 domain; WASLBP; 90 kDa SH3 protein interacting with Nck; 54 kDa vimentin-interacting protein; AF3P21; dia interacting protein; Diaphanous protein-interacting protein; diaphanous protein interacting protein; 54 kDa VacA-interacting protein; DIP; DIP1; DIP-1; NCK interacting protein with SH3 domain; anti-SPIN90 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SPIN90 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 672 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SPIN90 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody AAA211951 (lot AAA211951-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SPIN90 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody AAA211951 (lot AAA211951-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody AAA211951 (lot AAA211951-2) used for IP at 6 ug per reaction. SPIN90 was also immunoprecipitated by a previous lot of this antibody AAA211951-1. For blotting immunoprecipitated SPIN90, AAA211951 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody AAA211951 (lot AAA211951-2) used for IP at 6 ug per reaction. SPIN90 was also immunoprecipitated by a previous lot of this antibody AAA211951-1. For blotting immunoprecipitated SPIN90, AAA211951 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human SPIN90 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SPIN90 (Cat. No. AAA211951 Lot2) used at a dilution of 1:500 (0.4ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SPIN90 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SPIN90 (Cat. No. AAA211951 Lot2) used at a dilution of 1:500 (0.4ug/ml). Detection: DAB)
Related Product Information for anti-SPIN90 antibody
SPIN90 plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene encoding SPIN90, NCKIPSD, is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves NCKIPSD and the myeloid/lymphoid leukemia gene [taken from NCBI Entrez Gene (GeneID: 51517)].
Product Categories/Family for anti-SPIN90 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,196 Da
NCBI Official Full Name
Homo sapiens NCK interacting protein with SH3 domain (NCKIPSD), transcript variant 1, mRNA
NCBI Official Synonym Full Names
NCK interacting protein with SH3 domain
NCBI Official Symbol
NCKIPSD
NCBI Official Synonym Symbols
DIP; DIP1; ORF1; WISH; VIP54; AF3P21; SPIN90; WASLBP
NCBI Protein Information
NCK-interacting protein with SH3 domain; 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; WASP-interacting SH3-domain prote
UniProt Protein Name
NCK-interacting protein with SH3 domain
UniProt Gene Name
NCKIPSD
UniProt Synonym Gene Names
AF3P21; SPIN90; VIP54; DIP-1; WISH
UniProt Entry Name
SPN90_HUMAN
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Product Notes
The SPIN90 nckipsd (Catalog #AAA211951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SPIN90 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPIN90 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SPIN90 nckipsd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPIN90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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