WB (Western Blot)
(Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.)
Rabbit Sprouty 4 / SPRY4 Polyclonal Antibody | anti-SPRY4 antibody
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Gene Names
SPRY4; HH17
Reactivity
Chimpanzee, Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
Sprouty 4 / SPRY4, Antibody; Rabbit Polyclonal to Human Sprouty 4 / SPRY4; Anti-Sprouty 4 / SPRY4 Antibody (aa306-322) IHC-plus; SPRY4; Protein sprouty homolog 4; Sprouty 4; Sprouty homolog 4 (Drosophila); Spry-4; Human Sprouty 4; anti-SPRY4 antibody
Host
Rabbit
Reactivity
Chimpanzee, Human
Clonality
Polyclonal
Specificity
Amino acids 306-322 of Human Sprouty-4 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
322
Applicable Applications for anti-SPRY4 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human SPRY4.
Immunogen Type
Synthetic peptide
Immunogen
Sprouty 4 / SPRY4 antibody was raised against synthetic peptide from human SPRY4.
Antigen Modification
aa306-322
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.)
IHC (Immunohistochemistry)
(Anti-SPRY4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-SPRY4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-SPRY4 antibody
Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. Sprouty-4 is a cytoplasmic protein that interacts with the C-terminus of TESK1. Multiple splice variants have been reported for this protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,482 Da
NCBI Official Full Name
protein sprouty homolog 4 isoform 1
NCBI Official Synonym Full Names
sprouty homolog 4 (Drosophila)
NCBI Official Symbol
SPRY4
NCBI Official Synonym Symbols
HH17
NCBI Protein Information
protein sprouty homolog 4
UniProt Protein Name
Protein sprouty homolog 4
UniProt Gene Name
SPRY4
UniProt Synonym Gene Names
Spry-4
UniProt Entry Name
SPY4_HUMAN
Similar Products
Product Notes
The SPRY4 spry4 (Catalog #AAA50689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human Sprouty 4 / SPRY4 reacts with Chimpanzee, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sprouty 4 / SPRY4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SPRY4 spry4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sprouty 4 / SPRY4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.