IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SPRR1B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human SPRR1B Polyclonal Antibody | anti-SPRR1B antibody
SPRR1B Antibody (C-term)
Gene Names
SPRR1B; SPRR1; GADD33; CORNIFIN
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SPRR1B, Antibody; SPRR1B Antibody (C-term); Cornifin-B; 149 kDa pancornulin; Small proline-rich protein IB; SPR-IB; SPRR1B; anti-SPRR1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SPRR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-89 amino acids from the C-terminal region of human SPRR1B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.496 (varies by lot)
Sequence Positions
63-89
Sequence Length
89
Applicable Applications for anti-SPRR1B antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SPRR1B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SPRR1B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of SPRR1B Antibody (C-term) in MDA-MB231, MDA-MB435 cell line lysates (35ug/lane). SPRR1B (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SPRR1B Antibody (C-term) in MDA-MB231, MDA-MB435 cell line lysates (35ug/lane). SPRR1B (arrow) was detected using the purified Pab.)
Related Product Information for anti-SPRR1B antibody
SPRR1B is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
References
Stemmler,S., et.al., Int. J. Immunogenet. 36 (4), 217-222 (2009)
Kainu,K., et.al., Exp. Dermatol. 18 (2), 109-115 (2009)
Kainu,K., et.al., Exp. Dermatol. 18 (2), 109-115 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9888
NCBI Official Full Name
cornifin-B
NCBI Official Synonym Full Names
small proline-rich protein 1B
NCBI Official Symbol
SPRR1B
NCBI Official Synonym Symbols
SPRR1; GADD33; CORNIFIN
NCBI Protein Information
cornifin-B
UniProt Protein Name
Cornifin-B
UniProt Gene Name
SPRR1B
UniProt Synonym Gene Names
SPR-IB
UniProt Entry Name
SPR1B_HUMAN
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Product Notes
The SPRR1B sprr1b (Catalog #AAA285491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 63-89. The SPRR1B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPRR1B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPRR1B sprr1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPRR1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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