Rabbit SPT2 Polyclonal Antibody | anti-SPTLC2 antibody

SPT2 Antibody

Cat. Num. AAA39921

• Gene Names: SPTLC2; LCB2; SPT2; HSN1C; LCB2A; NSAN1C; hLCB2a
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: SPT2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SPT2, Antibody; SPT2 Antibody; SPT2; LCB2; HSN1C; LCB2A; NSAN1C; hLCB2a; KIAA0526; Serine palmitoyltransferase 2; Long chain base biosynthesis protein 2; LCB 2; serine palmitoyltransferase, long chain base subunit 2; anti-SPTLC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of SPT2 are known to exist; this antibody will not detect isoform b. SPT2 antibody is predicted to not cross-react with SPT1.
• Purity/Purification: SPT2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 562

• Applicable Applications for anti-SPTLC2 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: SPT2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SPT2.
• Buffer: SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SPT2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of SPT2 in 3T3 cells with SPT2 antibody at 20 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of SPT2 in 3T3 cells with SPT2 antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of SPT2 in 3T3 cell lysate with SPT2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-SPTLC2 antibody

SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

NCBI and Uniprot Product Information

• NCBI GI #: 4758668
• NCBI GeneID: 9517
• NCBI Accession #: NP_4854
• NCBI GenBank Nucleotide #: NM_004863.3
• UniProt Accession #: O15270; Q16685
• Molecular Weight: 62,924 Da
• NCBI Official Full Name: serine palmitoyltransferase 2
• NCBI Official Synonym Full Names: serine palmitoyltransferase, long chain base subunit 2
• NCBI Official Symbol: SPTLC2
• NCBI Official Synonym Symbols: LCB2; SPT2; HSN1C; LCB2A; NSAN1C; hLCB2a
• NCBI Protein Information: serine palmitoyltransferase 2; LCB 2; SPT 2; serine-palmitoyl-CoA transferase 2; long chain base biosynthesis protein 2a; serine palmitoyltransferase, subunit II
• UniProt Protein Name: Serine palmitoyltransferase 2
• UniProt Gene Name: SPTLC2
• UniProt Synonym Gene Names: KIAA0526; LCB2; LCB 2; LCB2a; SPT 2
• UniProt Entry Name: SPTC2_HUMAN