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IP (Immunoprecipitation)
(Immunoprecipitating SQSTM1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA231858 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA231858 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Rabbit anti-Human SQSTM1 Polyclonal Antibody | anti-SQSTM1 antibody
SQSTM1 Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
>95%, Protein G purified
Synonyms
SQSTM1, Antibody; SQSTM1 Antibody; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubi; SQSTM1; ORCA; OSIL; anti-SQSTM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
356
Applicable Applications for anti-SQSTM1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Species
Human
Immunogen
Recombinant human Sequestosome-1 protein
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10117 / sc-25575
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating SQSTM1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA231858 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA231858 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating SQSTM1 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA231858 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA231858 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of mcf-7 cells using AAA231858 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of mcf-7 cells using AAA231858 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA231858 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA231858 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human heart tissue using AAA231858 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human heart tissue using AAA231858 at dilution of 1:100)
Related Product Information for anti-SQSTM1 antibody
Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,629 Da
NCBI Official Full Name
sequestosome-1 isoform 2
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1
NCBI Official Synonym Symbols
p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
NCBI Protein Information
sequestosome-1
UniProt Protein Name
Sequestosome-1
UniProt Gene Name
SQSTM1
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60
UniProt Entry Name
SQSTM_HUMAN
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Product Notes
The SQSTM1 sqstm1 (Catalog #AAA231858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SQSTM1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SQSTM1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SQSTM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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