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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Rabbit SRC Polyclonal Antibody | anti-SRC antibody
SRC Antibody (Y419)
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Pig, Rat, Xenopus)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SRC, Antibody; SRC Antibody (Y419); Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC; SRC1; anti-SRC antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Pig, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
397-424
Sequence Length
542
Applicable Applications for anti-SRC antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with Phospho-SRC-Y419.ctrl, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with Phospho-SRC-Y419.ctrl, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of Phospho-SRC-Y419 in HepG2 cell line lysates (35ug/lane). SRC (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of Phospho-SRC-Y419 in HepG2 cell line lysates (35ug/lane). SRC (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419). AAA287338 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419). AAA287338 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-SRC antibody
SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Product Categories/Family for anti-SRC antibody
References
Muller,A.J., et.al., Mol. Cell. Biol. 12 (11), 5087-5093 (1992)
MacAuley,A., et.al., Oncogene 8 (1), 117-124 (1993)
den Hertog,J.,et.al., EMBO J. 12 (10), 3789-3798 (1993)
MacAuley,A., et.al., Oncogene 8 (1), 117-124 (1993)
den Hertog,J.,et.al., EMBO J. 12 (10), 3789-3798 (1993)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59835
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase Src
NCBI Official Synonym Full Names
SRC proto-oncogene, non-receptor tyrosine kinase
NCBI Official Symbol
SRC
NCBI Official Synonym Symbols
ASV; SRC1; c-SRC; p60-Src
NCBI Protein Information
proto-oncogene tyrosine-protein kinase Src
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase Src
UniProt Gene Name
SRC
UniProt Synonym Gene Names
SRC1; p60-Src
UniProt Entry Name
SRC_HUMAN
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Product Notes
The SRC src (Catalog #AAA287338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 397-424. The SRC Antibody (Y419) reacts with Human (Predicted Reactivity: Zebrafish, Bovine, Chicken, Mouse, Pig, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's SRC can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SRC src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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