WB (Western Blot)
(Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody AAA210896 (lot AAA210896-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human SRC1 Polyclonal Antibody | anti-SRC1 antibody
Rabbit anti-SRC1 Antibody, Affinity Purified
Gene Names
NCOA1; SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SRC1, Antibody; Rabbit anti-SRC1 Antibody, Affinity Purified; NCoA-1; steroid receptor coactivator-1; Steroid receptor coactivator 1; SRC-1; SRC1; RIP160; renal carcinoma antigen NY-REN-52; nuclear receptor coactivator 1; KAT13A; Hin-2 protein; F-SRC-1; class E basic helix-loop-helix protein 74; bHLHe74; bHLHe42; Protein Hin-2; Nuclear receptor coactivator 1; anti-SRC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SRC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1391 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody AAA210896 (lot AAA210896-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody AAA210896 (lot AAA210896-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody AAA210896 (lot AAA210896-2) used for IP at 6 ug per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210896-1). For blotting immunoprecipitated SRC1, AAA210896 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody AAA210896 (lot AAA210896-2) used for IP at 6 ug per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210896-1). For blotting immunoprecipitated SRC1, AAA210896 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SRC1 antibody
Steroid receptor coactivator 1 (SRC1) belongs to the SRC family of proteins that interacts with nuclear receptors to facilitate transcription. The 3 homologous members of this family are SRC-1 (NCoA-1), SRC-2 (GRIP1, TIF2, or NcoA-2), and SRC-3 (p/CIP, RAC3, ACTR, AIB1, or TRAM-1). SRC1contains histone acetyltransferase (HAT) activities and may facilitate transcription through chromatin remodeling. SRCs associate with p300/CBP-associated factor and CREB binding protein (CBP) as part of a multisubunit coactivating complex.
Product Categories/Family for anti-SRC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
156,628 Da
NCBI Official Full Name
Homo sapiens nuclear receptor coactivator 1 (NCOA1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
nuclear receptor coactivator 1
NCBI Official Symbol
NCOA1
NCBI Official Synonym Symbols
SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
NCBI Protein Information
nuclear receptor coactivator 1; Hin-2 protein; PAX3/NCOA1 fusion protein; class E basic helix-loop-helix protein 74; renal carcinoma antigen NY-REN-52; steroid receptor coactivator-1
UniProt Protein Name
Nuclear receptor coactivator 1
UniProt Gene Name
NCOA1
UniProt Synonym Gene Names
BHLHE74; SRC1; NCoA-1; bHLHe74; SRC-1
UniProt Entry Name
NCOA1_HUMAN
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Product Notes
The SRC1 ncoa1 (Catalog #AAA210896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SRC1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SRC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SRC1 ncoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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