FCM/FACS (Flow Cytometry)
(SRD5A3 Antibody (C-term) (AAA286540) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human SRD5A3 Polyclonal Antibody | anti-SRD5A3 antibody
SRD5A3 Antibody (C-term)
Gene Names
SRD5A3; CDG1P; CDG1Q; KRIZI; SRD5A2L; SRD5A2L1
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SRD5A3, Antibody; SRD5A3 Antibody (C-term); Polyprenol reductase; 3-oxo-5-alpha-steroid 4-dehydrogenase 3; Steroid 5-alpha-reductase 2-like; Steroid 5-alpha-reductase 3; S5AR 3; SR type 3; SRD5A3; SRD5A2L; anti-SRD5A3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SRD5A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human SRD5A3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
283-311
Sequence Length
318
Applicable Applications for anti-SRD5A3 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Function
Plays a key role in early steps of proteinN-linked glycosylation by being required forthe conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols intodolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Cellular Location
Endoplasmic reticulum membrane; Multi-passmembrane protein
Tissue Location
Overexpressed in hormone-refractoryprostate cancers (HRPC). Almost no or little expression in normal adult organs
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SRD5A3 Antibody (C-term) (AAA286540) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SRD5A3 Antibody (C-term) (AAA286540) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(SRD5A3 Antibody (C-term) (AAA286540) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SRD5A3 Antibody (C-term) (AAA286540) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SRD5A3 Antibody (C-term) (AAA286540) western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).This demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).)
WB (Western Blot)
(SRD5A3 Antibody (C-term) (AAA286540) western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).This demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).)
Related Product Information for anti-SRD5A3 antibody
SRD5A3 converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Product Categories/Family for anti-SRD5A3 antibody
References
Uemura, M., et al. Cancer Sci. 99(1):81-86(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36521
NCBI Official Full Name
polyprenol reductase
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 3
NCBI Official Symbol
SRD5A3
NCBI Official Synonym Symbols
CDG1P; CDG1Q; KRIZI; SRD5A2L; SRD5A2L1
NCBI Protein Information
polyprenol reductase
UniProt Protein Name
Polyprenol reductase
UniProt Gene Name
SRD5A3
UniProt Synonym Gene Names
SRD5A2L; S5AR 3; SR type 3
UniProt Entry Name
PORED_HUMAN
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Product Notes
The SRD5A3 srd5a3 (Catalog #AAA286540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 283-311. The SRD5A3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SRD5A3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SRD5A3 srd5a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRD5A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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