WB (Western Blot)
(Detection of human and mouse SRp55 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp55 antibody AAA212252 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody BL13266, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human, Mouse SRp55 Polyclonal Antibody | anti-SRp55 antibody
Rabbit anti-SRp55 Antibody, Affinity Purified
Gene Names
SRSF6; B52; SFRS6; SRP55; MGC5045; FLJ08061
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SRp55, Antibody; Rabbit anti-SRp55 Antibody, Affinity Purified; serine/arginine-rich splicing factor 6; SR splicing factor 6; splicing factor, arginine/serine-rich, 55 kDa; SRP55; SFRS6; HEL-S-91; epididymis secretory protein Li 91; B52; Splicing factor, arginine/serine-rich 6; pre-mRNA-splicing factor SRP55; Serine/arginine-rich splicing factor 6; serine and arginine rich splicing factor 6; anti-SRp55 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SRp55 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SRp55 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp55 antibody AAA212252 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody BL13266, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human and mouse SRp55 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp55 antibody AAA212252 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody BL13266, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of human SRp55 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SRp55 (Cat. No. AAA212252 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohiostchemistry)
(Detection of human SRp55 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SRp55 (Cat. No. AAA212252 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of mouse SRp55 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SRp55 (Cat. No. AAA212252 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse SRp55 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SRp55 (Cat. No. AAA212252 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-SRp55 antibody
Serine/arginine-rich protein 55 (SRp55) is encoded by the SRSF6 gene. SRp55 is involved in mRNA splicing and may play a role in the determination of alternative splicing. SRp55 belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12 [taken from NCBI Entrez Gene (Gene ID: 6431)].
Product Categories/Family for anti-SRp55 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,587 Da
NCBI Official Full Name
serine/arginine-rich splicing factor 6
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 6
NCBI Official Symbol
SRSF6
NCBI Official Synonym Symbols
B52; SFRS6; SRP55; MGC5045; FLJ08061
NCBI Protein Information
serine/arginine-rich splicing factor 6; OTTHUMP00000031020; SR splicing factor 6; pre-mRNA splicing factor SRP55; pre-mRNA-splicing factor SRP55; arginine/serine-rich splicing factor 6; splicing factor, arginine/serine-rich 6; splicing factor, arginine/se
UniProt Protein Name
Serine/arginine-rich splicing factor 6
UniProt Gene Name
SRSF6
UniProt Synonym Gene Names
SFRS6; SRP55
UniProt Entry Name
SRSF6_HUMAN
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Product Notes
The SRp55 srsf6 (Catalog #AAA212252) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SRp55 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SRp55 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SRp55 srsf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRp55, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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