IF (Immunofluorescence)
(Immunofluorescence staining of SRP72 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human SRP72 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human SRP72 Polyclonal Antibody | anti-SRP72 antibody
Anti-SRP72 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SRP72, Antibody; Anti-SRP72 Antibody, Rabbit Polyclonal; SRP72 Antibody, Rabbit PAb, Antigen Affinity Purified; signal recognition particle 72kDa; Anti-BMFF Antibody; Anti-BMFS1 Antibody; Anti-HEL103 Antibody; anti-SRP72 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SRP72
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SRP72 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human SRP72 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SRP72 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of SRP72 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human SRP72 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of SRP72 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human SRP72 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human SRP72 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human SRP72 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-SRP72 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:75 kDaObserved band size:75 kDa)
WB (Western Blot)
(Anti-SRP72 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:75 kDaObserved band size:75 kDa)
Related Product Information for anti-SRP72 antibody
SRP72 (Signal Recognition Particle 72) is a Protein Coding gene. The SRP72 gene, located on 4q12, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). Among the six proteins of the eukaryotic SRP, the largest protein, SRP72, is essential for protein targeting and possesses a poorly characterized RNA binding domain. SRP72 is widely expressed in the thyroid, lymph node, and other tissues. Diseases associated with SRP72 include Bone Marrow Failure Syndrome 1 and Anterior Spinal Artery Syndrome.
References
Iakhiaeva E, et al. (2010) Identification of amino acid residues in protein srp72 required for binding to a kinked 5e motif of the human signal recognition particle rna. BMC Mol Biol 11 83.Iakhiaeva E, et al. (2005) Identification of an rna-binding domain in human srp72. J Mol Biol 345 (4): 659-666.D'Altri T, et al. (2019) Heterozygous loss of srp72 in mice is not associated with major hematological phenotypes. Eur J Haematol 103 (4): 319-328.
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Product Notes
The SRP72 (Catalog #AAA256385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SRP72 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SRP72 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SRP72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRP72, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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