WB (Western Blot)
(Detection of human SSH1 by western blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SSH1 antibody AAA211410 (lot AAA211410-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SSH1 Polyclonal Antibody | anti-SSH1 antibody
Rabbit anti-SSH1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SSH1, Antibody; Rabbit anti-SSH1 Antibody, Affinity Purified; SSH1L; SSH-1L; protein phosphatase Slingshot homolog 1; hSSH-1L; SSH-like protein 1; slingshot homolog 1; Protein phosphatase Slingshot homolog 1; slingshot protein phosphatase 1; anti-SSH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SSH1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 999 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SSH1 by western blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SSH1 antibody AAA211410 (lot AAA211410-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SSH1 by western blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SSH1 antibody AAA211410 (lot AAA211410-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SSH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SSH1 antibody AAA211410 (lot AAA211410-2) used for IP at 6 ug per reaction. SSH1 was also immunoprecipitated by rabbit anti-SSH1 antibodies and A301-308A. For blotting immunoprecipitated SSH1, AAA211410 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SSH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SSH1 antibody AAA211410 (lot AAA211410-2) used for IP at 6 ug per reaction. SSH1 was also immunoprecipitated by rabbit anti-SSH1 antibodies and A301-308A. For blotting immunoprecipitated SSH1, AAA211410 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SSH1 antibody
SSH1, also known as slingshot, is a phosphatase that functions to activate the actin severing protein, ADF/cofilin, and influence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3. SSH1 has been shown to function downstream of alpha6beta4 integrin/Rac1 signaling in conjunction with 14-3-3 association.
Product Categories/Family for anti-SSH1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SSH1 ssh1 (Catalog #AAA211410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SSH1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSH1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SSH1 ssh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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