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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human SSH3 Polyclonal Antibody | anti-SSH3 antibody
SSH3 Antibody (C-term)
Gene Names
SSH3; SSH3L
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SSH3, Antibody; SSH3 Antibody (C-term); Protein phosphatase Slingshot homolog 3; SSH-like protein 3; SSH-3L; hSSH-3L; SSH3; SSH3L; anti-SSH3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.28 (varies by lot)
Sequence Positions
575-602
Sequence Length
141
Applicable Applications for anti-SSH3 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).)
WB (Western Blot)
(SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).)
Related Product Information for anti-SSH3 antibody
The ADF (actin-depolymerizing factor)/cofilin family (see
MIM 601442) is composed of stimulus-responsive mediators of actin
dynamics. ADF/cofilin proteins are inactivated by kinases such as
LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to
play a role in actin dynamics by reactivating ADF/cofilin proteins
in vivo (Niwa et al., 2002 [PubMed 11832213]).
MIM 601442) is composed of stimulus-responsive mediators of actin
dynamics. ADF/cofilin proteins are inactivated by kinases such as
LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to
play a role in actin dynamics by reactivating ADF/cofilin proteins
in vivo (Niwa et al., 2002 [PubMed 11832213]).
Product Categories/Family for anti-SSH3 antibody
References
Kousaka, K., et al. Genesis 46(5):246-255(2008)
Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)
Ohta, Y., et al. Genes Cells 8(10):811-824(2003)
Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)
Ohta, Y., et al. Genes Cells 8(10):811-824(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72996
NCBI Official Full Name
protein phosphatase Slingshot homolog 3
NCBI Official Synonym Full Names
slingshot protein phosphatase 3
NCBI Official Symbol
SSH3
NCBI Official Synonym Symbols
SSH3L
NCBI Protein Information
protein phosphatase Slingshot homolog 3
UniProt Protein Name
Protein phosphatase Slingshot homolog 3
UniProt Gene Name
SSH3
UniProt Synonym Gene Names
SSH3L; SSH-3L; hSSH-3L
UniProt Entry Name
SSH3_HUMAN
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Product Notes
The SSH3 ssh3 (Catalog #AAA287777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 575-602. The SSH3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSH3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SSH3 ssh3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSH3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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