FCM/FACS (Flow Cytometry)
(ST5 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ST5 Polyclonal Antibody | anti-ST5 antibody
ST5 Antibody (C-term)
Gene Names
ST5; HTS1; p126; DENND2B
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ST5, Antibody; ST5 Antibody (C-term); Suppression of tumorigenicity 5 protein; DENN domain-containing protein 2B; HeLa tumor suppression 1; ST5; DENND2B; HTS1; anti-ST5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1106-1137 amino acids from the C-terminal region of human ST5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.34 (varies by lot)
Sequence Positions
1106-1137
Sequence Length
609
Applicable Applications for anti-ST5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ST5 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ST5 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ST5 Antibody (C-term K1121) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).)
WB (Western Blot)
(ST5 Antibody (C-term K1121) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).)
Related Product Information for anti-ST5 antibody
ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.
Product Categories/Family for anti-ST5 antibody
References
Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000).
Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999).
Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998).
Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996).
Lichy, J.H., et al., Cell Growth Differ. 3(8):541-548 (1992).
Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999).
Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998).
Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996).
Lichy, J.H., et al., Cell Growth Differ. 3(8):541-548 (1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126485
NCBI Official Full Name
suppression of tumorigenicity 5 protein isoform 1
NCBI Official Synonym Full Names
suppression of tumorigenicity 5
NCBI Official Symbol
ST5
NCBI Official Synonym Symbols
HTS1; p126; DENND2B
NCBI Protein Information
suppression of tumorigenicity 5 protein
UniProt Protein Name
Suppression of tumorigenicity 5 protein
UniProt Gene Name
ST5
UniProt Synonym Gene Names
DENND2B; HTS1
UniProt Entry Name
ST5_HUMAN
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Product Notes
The ST5 st5 (Catalog #AAA288966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1106-1137. The ST5 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ST5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ST5 st5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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