WB (Western Blot)
(STK35 Antibody (C255) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the STK35 antibody detected the STK35 protein (arrow).)
Rabbit anti-Human, mouse STK35 Polyclonal Antibody | anti-STK35 antibody
STK35 Antibody (Center)
Gene Names
STK35; CLIK1; STK35L1
Reactivity
Human, mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STK35, Antibody; STK35 Antibody (Center); Serine/threonine-protein kinase 35; CLP-36-interacting kinase 1; CLIK-1; PDLIM1-interacting kinase 1; Serine/threonine-protein kinase 35 L1; STK35; CLIK1; PDIK1; STK35L1; anti-STK35 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STK35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-404 amino acids from the Central region of human STK35.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
374-404
Sequence Length
534
Applicable Applications for anti-STK35 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Calculated MW
58051
Cellular location
Nucleus. Nucleus, nucleolus. Cytoplasm. Note=When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only inthe nucleus, and the presence of PDLIM1 had no influence on its location
Tissue location
Expressed in testis.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(STK35 Antibody (C255) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the STK35 antibody detected the STK35 protein (arrow).)
WB (Western Blot)
(STK35 Antibody (C255) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the STK35 antibody detected the STK35 protein (arrow).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-STK35 Pab is used in Western blot to detect STK35 in mouse kidney tissue lysate.)
WB (Western Blot)
(The anti-STK35 Pab is used in Western blot to detect STK35 in mouse kidney tissue lysate.)
Related Product Information for anti-STK35 antibody
STK35 can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded kinase may be a regulator of actin stress fibers in nonmuscle cells. This protein has also been found in the nucleus.
Product Categories/Family for anti-STK35 antibody
References
Vallenius, T., et al., J. Cell. Sci. 115 (Pt 10), 2067-2073 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase 35
NCBI Official Synonym Full Names
serine/threonine kinase 35
NCBI Official Symbol
STK35
NCBI Official Synonym Symbols
CLIK1; STK35L1
NCBI Protein Information
serine/threonine-protein kinase 35
UniProt Protein Name
Serine/threonine-protein kinase 35
UniProt Gene Name
STK35
UniProt Synonym Gene Names
CLIK1; PDIK1; STK35L1; CLIK-1
UniProt Entry Name
STK35_HUMAN
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Product Notes
The STK35 stk35 (Catalog #AAA286292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 374-404. The STK35 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STK35 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the STK35 stk35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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