Rabbit SUMO2/3 Polyclonal Antibody | anti-SUMO3 antibody

SUMO2/3 Antibody (C-term)

Cat. Num. AAA28663

• Gene Names: SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
• Reactivity: Human, mouse (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Pig, Rat)
• Applications: Immunofluorescence, ELISA, Western Blot, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SUMO2/3, Antibody; SUMO2/3 Antibody (C-term); Small ubiquitin-related modifier 3; SUMO-3; SMT3 homolog 1 {ECO:0000312|HGNC:HGNC:11124}; SUMO-2; Ubiquitin-like protein SMT3A; Smt3A; SUMO3 HGNC:11124); anti-SUMO3 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 49-81
• Sequence Length: 103

• Applicable Applications for anti-SUMO3 antibody: Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF~~1:25; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)

WB (Western Blot)

(SUMO2/3 Antibody (C-term) western blot analysis in Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).)

WB (Western Blot)

(SUMO2/3 Antibody (C-term) western blot analysis in U251 cell lysate,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates that the SUMO2/3 antibody detected SUMO2/3 protein (arrow).)

WB (Western Blot)

(Western blot analysis of lysates from 293T, Hela, HL-60, Jurkat cell lines and rat liver tissue lysate (from left to right), using SUMO2/3 Antibody (Q65). AAA28663 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IF (Immunofluorescence)

(Fluorescent image of U251 cells stained with SUMO2/3 Antibody(C-term). AAA28663 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IF (Immunofluorescence)

(Fluorescent image of Hela cells stained with SUMO2/3 Antibody (C-term). AAA28663 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IF (Immunofluorescence)

(Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). AAA28663 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Related Product Information for anti-SUMO3 antibody

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Product Categories/Family for anti-SUMO3 antibody

Crown Antibodies; Cell Biology

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Lapenta, V., et al., Genomics 40(2):362-366 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 48928058
• NCBI GeneID: 6612
• NCBI Accession #: NP_008867.2
• NCBI GenBank Nucleotide #: NM_006936.2
• UniProt Accession #: P55854; Q53HI9; Q6FGD4; Q9BWR4; B2R5X4; B4DUW4
• Molecular Weight: 11637
• NCBI Official Full Name: small ubiquitin-related modifier 3 isoform 1
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 3
• NCBI Official Symbol: SUMO3
• NCBI Official Synonym Symbols: SMT3A; Smt3B; SMT3H1; SUMO-3
• NCBI Protein Information: small ubiquitin-related modifier 3
• UniProt Protein Name: Small ubiquitin-related modifier 3
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: Smt3A
• UniProt Entry Name: SUMO3_HUMAN