Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Human.)
Rabbit anti-Human, Pig Superoxide Dismutase 2, Mitochondrial (SOD2) Polyclonal Antibody | anti-SOD2 antibody
Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Reactivity
Human, Pig
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Superoxide Dismutase 2, Mitochondrial (SOD2), Antibody; Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2); anti-SOD2 antibody
Host
Rabbit
Reactivity
Human, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SOD2. It has been selected for its ability to recognize SOD2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEAIKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK
MGHHHHHHSGSEF-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEAIKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK
Sequence Length
222
Applicable Applications for anti-SOD2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB), IP, IF, FCM.
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Superoxide Dismutase 2, Mitochondrial (SOD2)
Cross Reactivity
Human, Pig
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Human.)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: SOD2 knockout MCF7 cell lysate; Predicted MW: 18,20,21,25kd Observed MW: 21kd Primary Ab: 3ug/ml Rabbit Anti-Human SOD2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Human.)
WB (Western Blot)
(Western Blot: Sample. Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human HeLa Cells; Lane4: Porcine Brain Tissue; Lane5: Human MCF-7 Cells.)
WB (Western Blot)
(Western Blot: Sample. Lane1: Human Serum; Lane2: Porcine Liver Tissue; Lane3: Human HeLa Cells; Lane4: Porcine Brain Tissue; Lane5: Human MCF-7 Cells.)
Product Categories/Family for anti-SOD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,730 Da
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 2
NCBI Official Symbol
SOD2
NCBI Official Synonym Symbols
IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
NCBI Protein Information
superoxide dismutase [Mn], mitochondrial
UniProt Protein Name
Superoxide dismutase [Mn], mitochondrial
UniProt Gene Name
SOD2
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Product Notes
The SOD2 sod2 (Catalog #AAA20109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2) reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 2, Mitochondrial (SOD2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB), IP, IF, FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the SOD2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVTEEKYQ EALAKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPKGELLEA IKRDFGSFDK FKEKLTAASV GVQGSGWGWL GFNKERGHLQ IAACPNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTERYMAC KK. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 2, Mitochondrial (SOD2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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