Porcine Superoxide Dismutase 2, Mitochondrial ELISA Kit | SOD2 elisa kit

Porcine Superoxide Dismutase 2, Mitochondrial ELISA Kit

Cat. Num. AAA88476

• Gene Names: SOD2; IPOB; MNSOD; MVCD6
• Reactivity: Porcine
• Synonyms: Superoxide Dismutase 2, Mitochondrial; N/A; Porcine Superoxide Dismutase 2, Mitochondrial ELISA Kit; SOD2 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOD2. No significant cross-reactivity or interference between SOD2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SOD2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for SOD2 elisa kit

Principle of the Assay: SOD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SOD2 antibody and an SOD2-HRP conjugate. The assay sample and buffer are incubated together with SOD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD2 concentration since SOD2 from samples and SOD2-HRP conjugate compete for the anti-SOD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD2 from the sample, fewer sites are left to bind SOD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This SOD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SOD2. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 15214595
• NCBI GeneID: 6648
• Molecular Weight: 19,730 Da
• NCBI Official Full Name: Superoxide dismutase 2, mitochondrial
• NCBI Official Synonym Full Names: superoxide dismutase 2, mitochondrial
• NCBI Official Symbol: SOD2
• NCBI Official Synonym Symbols: IPOB; MNSOD; MVCD6
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• UniProt Gene Name: SOD2
• UniProt Entry Name: SODM_HUMAN