WB (Western Blot)
(Suz12 antibody (pAb) tested by Western blot. HeLa cell nuclear extract (20 ?g) was probed with Suz12 pAb (1:500 dilution).)
Rabbit anti-Human, Mouse Suz12 Polyclonal Antibody | anti-Suz12 antibody
Suz12 antibody (pAb)
Gene Names
SUZ12; CHET9; JJAZ1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Suz12, Antibody; Suz12 antibody (pAb); CHET9; JJAZ1; anti-Suz12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Suz12 antibody
WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Suz12 antibody (pAb) tested by Western blot. HeLa cell nuclear extract (20 ?g) was probed with Suz12 pAb (1:500 dilution).)
WB (Western Blot)
(Suz12 antibody (pAb) tested by Western blot. HeLa cell nuclear extract (20 ?g) was probed with Suz12 pAb (1:500 dilution).)
ChIP (Chromatin Immunoprecipitation)
(Suz12 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human non-small cell lung carcinoma cell line NCI-H1299 cells and 10 ul of Suz12 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(Suz12 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human non-small cell lung carcinoma cell line NCI-H1299 cells and 10 ul of Suz12 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(Suz12 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human B cell lymphoma cell line and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Suz12 binding sites. The image shows binding across a region of chromosome 12.)
ChIP (Chromatin Immunoprecipitation)
(Suz12 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human B cell lymphoma cell line and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Suz12 binding sites. The image shows binding across a region of chromosome 12.)
Related Product Information for anti-Suz12 antibody
Short Description: Our Suz12 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.
Background: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.
Product Categories/Family for anti-Suz12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 81 kDa
Observed: 96 kDa
Observed: 96 kDa
NCBI Official Full Name
polycomb protein SUZ12
NCBI Official Synonym Full Names
SUZ12 polycomb repressive complex 2 subunit
NCBI Official Symbol
SUZ12
NCBI Official Synonym Symbols
CHET9; JJAZ1
NCBI Protein Information
polycomb protein SUZ12; chET 9 protein; joined to JAZF1 protein; suppressor of zeste 12 homolog; suppressor of zeste 12 protein homolog; chromatin precipitated E2F target 9 protein
UniProt Protein Name
Polycomb protein SUZ12
UniProt Gene Name
SUZ12
UniProt Synonym Gene Names
CHET9; JJAZ1; KIAA0160; ChET 9 protein
UniProt Entry Name
SUZ12_HUMAN
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Product Notes
The Suz12 suz12 (Catalog #AAA59807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Suz12 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Suz12 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Suz12 suz12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Suz12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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