WB (Western Blot)
(Western blot analysis of Phospho-Syntaxin 1A (Ser14) expression in various lysates)
Rabbit Syntaxin 1A Polyclonal Antibody | anti-STX1A antibody
Phospho-Syntaxin 1A (Ser14) Antibody
Gene Names
STX1A; STX1; HPC-1; P35-1; SYN1A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
Syntaxin 1A, Antibody; Phospho-Syntaxin 1A (Ser14) Antibody; HPC 1; Neuron specific antigen HPC1; Neuron-specific antigen HPC-1; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; P35-1; STX1; STX1A; STX1A_HUMAN; SYN1A; Syntaxin 1A (brain); Syntaxin 1A brain; Syntaxin-1A; anti-STX1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Syntaxin 1A (Ser14) antibody detects endogenous levels of Syntaxin 1A only when phosphorylated at Serine 14
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
251
Applicable Applications for anti-STX1A antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Serine 14
Subcellular Location
Secreted and Cytoplasmic Vesicle > Secretory Vesicle > Synaptic Vesicle Membrane. Cell Junction > Synapse > Synaptosome.
Tissue Specificity
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Predicted Cross Reactivity
Bovine, Sheep
Similarity
Bovine (100%), Sheep (100%)
Conjugation
Unconjugated
Epitope
Phospho Ser14
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-Syntaxin 1A (Ser14) expression in various lysates)
WB (Western Blot)
(Western blot analysis of Phospho-Syntaxin 1A (Ser14) expression in various lysates)
IF (Immunofluorescence)
(AAA321751 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321751 staining NIH/3T3 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of Syntaxin 1A phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Syntaxin 1A phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-STX1A antibody
Description: STX1A is a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described.
Function: Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.
Subunit Structure: Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A (By similarity). This complex binds to CPLX1 (By similarity). Binds SYTL4 and STXBP6 (By similarity). Found in a ternary complex with STX1A and SNAP25 (By similarity). Interacts with PLCL1 (via C2 domain) (By similarity). Interacts with OTOF and LGI3 (By similarity). Interacts with SLC6A4 (By similarity). Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent (By similarity). Found in a complex with VAMP8 and SNAP23 (PubMed:12130530). Interacts with VAPA and SYBU (PubMed:15459722). Interacts with STXBP1 and DAPK1 (PubMed:12730201). Interacts with PRRT2 (By similarity).
Post-translational Modifications: Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.
Similarity: Belongs to the syntaxin family.
Function: Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.
Subunit Structure: Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A (By similarity). This complex binds to CPLX1 (By similarity). Binds SYTL4 and STXBP6 (By similarity). Found in a ternary complex with STX1A and SNAP25 (By similarity). Interacts with PLCL1 (via C2 domain) (By similarity). Interacts with OTOF and LGI3 (By similarity). Interacts with SLC6A4 (By similarity). Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent (By similarity). Found in a complex with VAMP8 and SNAP23 (PubMed:12130530). Interacts with VAPA and SYBU (PubMed:15459722). Interacts with STXBP1 and DAPK1 (PubMed:12730201). Interacts with PRRT2 (By similarity).
Post-translational Modifications: Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.
Similarity: Belongs to the syntaxin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 35 kDa
Predicted: 34 kDa
Predicted: 34 kDa
NCBI Official Full Name
syntaxin-1A isoform 2
NCBI Official Synonym Full Names
syntaxin 1A
NCBI Official Symbol
STX1A
NCBI Official Synonym Symbols
STX1; HPC-1; P35-1; SYN1A
NCBI Protein Information
syntaxin-1A
UniProt Protein Name
Syntaxin-1A
UniProt Gene Name
STX1A
UniProt Synonym Gene Names
STX1
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Product Notes
The STX1A stx1a (Catalog #AAA321751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Syntaxin 1A (Ser14) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Syntaxin 1A can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the STX1A stx1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syntaxin 1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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