FCM/FACS (Flow Cytometry)
(TAF4B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TAF4B Polyclonal Antibody | anti-TAF4B antibody
TAF4B Antibody (C-term)
Gene Names
TAF4B; SPGF13; TAF2C2; TAFII105
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TAF4B, Antibody; TAF4B Antibody (C-term); Transcription initiation factor TFIID subunit 4B; Transcription initiation factor TFIID 105 kDa subunit; TAF(II)105; TAFII-105; TAFII105; TAF4B; TAF2C2; anti-TAF4B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TAF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-675 amino acids from the C-terminal region of human TAF4B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.37 (varies by lot)
Sequence Positions
647-675
Sequence Length
862
Applicable Applications for anti-TAF4B antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TAF4B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TAF4B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(TAF4B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TAF4B antibody detected the TAF4B protein (arrow).)
WB (Western Blot)
(TAF4B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TAF4B antibody detected the TAF4B protein (arrow).)
Related Product Information for anti-TAF4B antibody
TATA-binding protein associated factors (TAFs)
participate, with TATA binding protein (TBP; MIM 600075), in the
formation of the TFIID protein complex (see MIM 313650), which is
involved in the initiation of gene transcription by RNA polymerase
II (see MIM 180660).
participate, with TATA binding protein (TBP; MIM 600075), in the
formation of the TFIID protein complex (see MIM 313650), which is
involved in the initiation of gene transcription by RNA polymerase
II (see MIM 180660).
References
Kalogeropoulou, M., et al. Mol. Cancer Res. 8(4):554-568(2010)
Gazit, K., et al. J. Biol. Chem. 284(39):26286-26296(2009)
Knauff, E.A., et al. Hum. Reprod. 24(9):2372-2378(2009)
Teye, K., et al. Int. J. Oncol. 33(6):1271-1280(2008)
Liu, W.L., et al. Mol. Cell 29(1):81-91(2008)
Gazit, K., et al. J. Biol. Chem. 284(39):26286-26296(2009)
Knauff, E.A., et al. Hum. Reprod. 24(9):2372-2378(2009)
Teye, K., et al. Int. J. Oncol. 33(6):1271-1280(2008)
Liu, W.L., et al. Mol. Cell 29(1):81-91(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91091
NCBI Official Full Name
transcription initiation factor TFIID subunit 4B isoform 2
NCBI Official Synonym Full Names
TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa
NCBI Official Symbol
TAF4B
NCBI Official Synonym Symbols
SPGF13; TAF2C2; TAFII105
NCBI Protein Information
transcription initiation factor TFIID subunit 4B
UniProt Protein Name
Transcription initiation factor TFIID subunit 4B
UniProt Gene Name
TAF4B
UniProt Synonym Gene Names
TAF2C2; TAFII105; TAF(II)105; TAFII-105; TAFII105
UniProt Entry Name
TAF4B_HUMAN
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Product Notes
The TAF4B taf4b (Catalog #AAA286275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 647-675. The TAF4B Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TAF4B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TAF4B taf4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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