IF (Immunofluorescence)
(Immunofluorescence staining of TATDN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TATDN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human TATDN1 Polyclonal Antibody | anti-TATDN1 antibody
Anti-TATDN1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
TATDN1, Antibody; Anti-TATDN1 Antibody, Rabbit Polyclonal; TATDN1 Antibody, Rabbit PAb, Antigen Affinity Purified; TatD DNase domain containing 1; Anti-CDA11 Antibody; anti-TATDN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TATDN1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TATDN1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human TATDN1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TATDN1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of TATDN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TATDN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of TATDN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TATDN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TATDN1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TATDN1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TATDN1 antibody
The DNase domain-containing protein TATDN1 is a conserved nuclease in both prokaryotes and eukaryotes. It was previously implicated to play a role in apoptotic DNA fragmentation in yeast and C. elegans. zebrafish TATDN1 (zTATDN1) plays an important role in chromosome segregation and eye development in zebrafish. TATDN1 promotes the proliferative potential and cell cycle progression of breast cancer (BCa) cells through adsorbing microRNA-140-3p to upregulate NOVA1 expression. TATDN1 accelerates the proliferative rate and cell cycle progression of hepatocellular carcinoma (HCC) by degrading miRNA-6089 to upregulate LIX1L. TATDN1 enhanced the DDP-tolerance of non-small cell lung cancer (NSCLC) cells by upregulating TRIM66 expression via sponging miR-451, hinting at a novel regulatory pathway of chemoresistance in DDP-tolerant NSCLC cells and providing a potential therapeutic target for NSCLC patients with DDP-resistance.
References
Yu XY, et al. (2019) Tatdn1 promotes the development and progression of breast cancer by targeting microrna-140-3p. Eur Rev Med Pharmacol Sci 23 (12): 5293-5300.Yang H, et al. (2012) The dnase domain-containing protein tatdn1 plays an important role in chromosomal segregation and cell cycle progression during zebrafish eye development. Cell Cycle 11 (24): 4626-4632.Wang L, et al. (2019) Lncrna tatdn1 contributes to the cisplatin resistance of non-small cell lung cancer through tatdn1/mir-451/trim66 axis. Cancer Biol Ther 20 (3): 261-271.Shen C, et al. (2019) Lncrna tatdn1 induces the progression of hepatocellular carcinoma via targeting mirna-6089. Eur Rev Med Pharmacol Sci 23 (15): 6459-6466.
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Product Notes
The TATDN1 (Catalog #AAA256653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TATDN1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TATDN1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TATDN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TATDN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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