FCM/FACS (Flow Cytometry)
(TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TBC1D4 Polyclonal Antibody | anti-TBC1D4 antibody
TBC1D4 Antibody (N-term)
Gene Names
TBC1D4; AS160; NIDDM5
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TBC1D4, Antibody; TBC1D4 Antibody (N-term); TBC1 domain family member 4; Akt substrate of 160 kDa; AS160; TBC1D4; KIAA0603; anti-TBC1D4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TBC1D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-201 amino acids from the N-terminal region of human TBC1D4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
172-201
Sequence Length
515
Applicable Applications for anti-TBC1D4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of TBC1D4 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). TBC1D4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of TBC1D4 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). TBC1D4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-TBC1D4 antibody
TBC1D4 may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Product Categories/Family for anti-TBC1D4 antibody
References
Lee,S.Y., et.al., Proc. Natl. Acad. Sci. U.S.A. 100 (5), 2651-2656 (2003)
Nakayama,M., et.al., Genome Res. 12 (11), 1773-1784 (2002)
Nakayama,M., et.al., Genome Res. 12 (11), 1773-1784 (2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
146563
NCBI Official Full Name
TBC1 domain family member 4 isoform 1
NCBI Official Synonym Full Names
TBC1 domain family, member 4
NCBI Official Symbol
TBC1D4
NCBI Official Synonym Symbols
AS160; NIDDM5
NCBI Protein Information
TBC1 domain family member 4
UniProt Protein Name
TBC1 domain family member 4
UniProt Gene Name
TBC1D4
UniProt Synonym Gene Names
AS160; KIAA0603; AS160
UniProt Entry Name
TBCD4_HUMAN
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Product Notes
The TBC1D4 tbc1d4 (Catalog #AAA289380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 172-201. The TBC1D4 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TBC1D4 tbc1d4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBC1D4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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