Rabbit anti-Human TBCE Polyclonal Antibody | anti-TBCE antibody

Anti-TBCE Antibody, Rabbit Polyclonal

Cat. Num. AAA256654

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: TBCE, Antibody; Anti-TBCE Antibody, Rabbit Polyclonal; TBCE Antibody, Rabbit PAb, Antigen Affinity Purified; tubulin-specific chaperone E; Anti-HRD Antibody; Anti-KCS Antibody; Anti-KCS1 Antibody; Anti-pac2 Antibody; anti-TBCE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TBCE
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-TBCE antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human TBCE fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human TBCE fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(TBCE was immunoprecipitated using:Lane A:0.5 mg Jurkat HeLa Whole Cell Lysate4 uL anti-TBCE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TBCE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :55 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human TBCE in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-TBCE rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:59 kDaObserved band size:55 kDa)

Related Product Information for anti-TBCE antibody

Tubulin-folding cofactor E (TBCE) is an alpha-tubulin-binding protein involved in the formation of the tubulin dimer and microtubule dynamics, through the regulation of tubulin heterodimer dissociation. TBCE has also been implicated in two important related human disorders, the Kenny-Caffey and Sanjad-Sakati syndromes. The expression of TBCE as a recombinant protein in bacteria results in the formation of insoluble inclusion bodies in the absence of denaturing agents. Hypoparathyroidism, mental retardation, and facial dysmorphism (HRD) is a fatal developmental disease caused by mutations in TBCE. The gene defect in the pmn/pmn mouse is localized to a missense mutation in the tubulin-specific chaperone E (TBCE) gene on mouse chromosome 13, which is one of the five tubulin-specific chaperons involved in tubulin folding and dimerization.

References

Touati A, et al. (2019) Additional tunisian patients with sanjad-sakati syndrome: A review toward a consensus on diagnostic criteria. Arch Pediatr 26 (2): 102-107.Rak K, et al. (2013) Mutation of the tbce gene causes disturbance of microtubules in the auditory nerve and cochlear outer hair cell degeneration accompanied by progressive hearing loss in the pmn/pmn mouse. Exp Neurol 250 333-340.Kortazar D, et al. (2006) Native tubulin-folding cofactor e purified from baculovirus-infected sf9 cells dissociates tubulin dimers. Protein Expr Purif 49 (2): 196-202.Jin S, et al. (2009) Drosophila tubulin-specific chaperone e functions at neuromuscular synapses and is required for microtubule network formation. Development 136 (9): 1571-1581.