IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.)
Rabbit TBX6 Polyclonal Antibody | anti-TBX6 antibody
TBX6 Antibody (Center)
Gene Names
TBX6; SCDO5
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TBX6, Antibody; TBX6 Antibody (Center); T-box transcription factor TBX6; T-box protein 6; TBX6; anti-TBX6 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
264-293
Sequence Length
436
Applicable Applications for anti-TBX6 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.)
WB (Western Blot)
(TBX6 Antibody (Center) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).)
WB (Western Blot)
(TBX6 Antibody (Center) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).)
Related Product Information for anti-TBX6 antibody
This gene is a member of a phylogenetically conserved
family of genes that share a common DNA-binding domain, the T-box.
T-box genes encode transcription factors involved in the regulation
of developmental processes. Knockout studies in mice indicate that
this gene is important for specification of paraxial mesoderm
structures.
family of genes that share a common DNA-binding domain, the T-box.
T-box genes encode transcription factors involved in the regulation
of developmental processes. Knockout studies in mice indicate that
this gene is important for specification of paraxial mesoderm
structures.
Product Categories/Family for anti-TBX6 antibody
References
Fei, Q., et al. Spine 35(9):983-988(2010)
Ghebranious, N., et al. J. Bone Miner. Res. 23(10):1576-1583(2008)
Farin, H.F., et al. J. Biol. Chem. 282(35):25748-25759(2007)
Papapetrou, C., et al. Genomics 55(2):238-241(1999)
Yi, C.H., et al. Genomics 55(1):10-20(1999)
Ghebranious, N., et al. J. Bone Miner. Res. 23(10):1576-1583(2008)
Farin, H.F., et al. J. Biol. Chem. 282(35):25748-25759(2007)
Papapetrou, C., et al. Genomics 55(2):238-241(1999)
Yi, C.H., et al. Genomics 55(1):10-20(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47045
NCBI Official Full Name
T-box transcription factor TBX6
NCBI Official Synonym Full Names
T-box 6
NCBI Official Symbol
TBX6
NCBI Official Synonym Symbols
SCDO5
NCBI Protein Information
T-box transcription factor TBX6
UniProt Protein Name
T-box transcription factor TBX6
UniProt Gene Name
TBX6
UniProt Synonym Gene Names
T-box protein 6
UniProt Entry Name
TBX6_HUMAN
Similar Products
Product Notes
The TBX6 tbx6 (Catalog #AAA285959) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 264-293. The TBX6 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TBX6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TBX6 tbx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.