FCM/FACS (Flow Cytometry)
(TCF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TCF4 Polyclonal Antibody | anti-TCF4 antibody
TCF4 Antibody (N-term)
Gene Names
TCF4; E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TCF4, Antibody; TCF4 Antibody (N-term); Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF4; BHLHB19; ITF2; SEF2; anti-TCF4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-73 amino acids from the N-terminal region of human TCF4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
48-73
Sequence Length
671
Applicable Applications for anti-TCF4 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TCF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TCF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(TCF4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TCF4 antibody detected the TCF4 protein (arrow).)
WB (Western Blot)
(TCF4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TCF4 antibody detected the TCF4 protein (arrow).)
Related Product Information for anti-TCF4 antibody
TCF4 is transcription factor 4, a basic
helix-turn-helix transcription factor. The encoded protein
recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a
motif first identified in immunoglobulin enhancers. This gene is
expressed predominantly in pre-B-cells, although it is found in
other tissues as well. Defects in this gene are a cause of
Pitt-Hopkins syndrome.
helix-turn-helix transcription factor. The encoded protein
recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a
motif first identified in immunoglobulin enhancers. This gene is
expressed predominantly in pre-B-cells, although it is found in
other tissues as well. Defects in this gene are a cause of
Pitt-Hopkins syndrome.
Product Categories/Family for anti-TCF4 antibody
References
Rosenfeld, J.A., et al. Genet. Med. 11(11):797-805(2009)
Herbst, A., et al. Biochem. Biophys. Res. Commun. 387(4):736-740(2009)
Rena, V., et al. Placenta 30(10):876-883(2009)
Henthorn, P., et al. Science 247(4941):467-470(1990)
Herbst, A., et al. Biochem. Biophys. Res. Commun. 387(4):736-740(2009)
Rena, V., et al. Placenta 30(10):876-883(2009)
Henthorn, P., et al. Science 247(4941):467-470(1990)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71308
NCBI Official Full Name
transcription factor 4 isoform a
NCBI Official Synonym Full Names
transcription factor 4
NCBI Official Symbol
TCF4
NCBI Official Synonym Symbols
E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
NCBI Protein Information
transcription factor 4
UniProt Protein Name
Transcription factor 4
UniProt Gene Name
TCF4
UniProt Synonym Gene Names
BHLHB19; ITF2; SEF2; TCF-4; bHLHb19; ITF-2; SEF-2
UniProt Entry Name
ITF2_HUMAN
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Product Notes
The TCF4 tcf4 (Catalog #AAA287019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 48-73. The TCF4 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's TCF4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TCF4 tcf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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