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WB (Western Blot)
(Detection of human TDG by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TDG antibody AAA212647 (lot AAA212647-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human TDG Polyclonal Antibody | anti-TDG antibody
Rabbit anti-TDG Antibody, Affinity Purified
Gene Names
TDG; hTDG
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TDG, Antibody; Rabbit anti-TDG Antibody, Affinity Purified; G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase; thymine DNA glycosylase; anti-TDG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TDG antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 360 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TDG by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TDG antibody AAA212647 (lot AAA212647-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human TDG by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TDG antibody AAA212647 (lot AAA212647-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TDG by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TDG antibody AAA212647 (lot AAA212647-1) used for IP at 6 ug per reaction. TDG was also immunoprecipitated by rabbit anti-TDG antibody BL16331. For blotting immunoprecipitated TDG, AAA212647 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TDG by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TDG antibody AAA212647 (lot AAA212647-1) used for IP at 6 ug per reaction. TDG was also immunoprecipitated by rabbit anti-TDG antibody BL16331. For blotting immunoprecipitated TDG, AAA212647 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human TDG by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-TDG (Cat. No. AAA212647 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human TDG by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-TDG (Cat. No. AAA212647 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TDG by immunohistochemistry. Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti-TDG (Cat. No. AAA212647 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TDG by immunohistochemistry. Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti-TDG (Cat. No. AAA212647 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-TDG antibody
Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].
Product Categories/Family for anti-TDG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,053 Da
NCBI Official Full Name
G/T mismatch-specific thymine DNA glycosylase
NCBI Official Synonym Full Names
thymine-DNA glycosylase
NCBI Official Symbol
TDG
NCBI Official Synonym Symbols
hTDG
NCBI Protein Information
G/T mismatch-specific thymine DNA glycosylase
UniProt Protein Name
G/T mismatch-specific thymine DNA glycosylase
UniProt Gene Name
TDG
UniProt Synonym Gene Names
hTDG
UniProt Entry Name
TDG_HUMAN
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Product Notes
The TDG tdg (Catalog #AAA212647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TDG Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TDG can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TDG tdg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TDG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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