Rabbit TECTA Polyclonal Antibody | anti-TECTA antibody

Anti-TECTA Antibody

Cat. Num. AAA11686

• Gene Names: TECTA; DFNA8; DFNA12; DFNB21
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: TECTA, Antibody; Anti-TECTA Antibody; Alpha-tectorin; tectorin alpha; anti-TECTA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 2155

• Applicable Applications for anti-TECTA antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Ms, Rat; ; Immunohistochemistry(IHC) (Paraffin-embedded Section:; Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.; Optimal dilutions should be determined by end users.; ; Antibody can be supported by chemiluminescence kit
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TECTA (93-134aa RAFVAPFWADVHNGIRGEIYYRETMEPAILKRATKDIRKYFK), different from the related mouse sequence by three amino acids.
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(TECTA was detected in paraffin-embedded sections of human testis tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry)

(TECTA was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry)

(TECTA was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot)

(Western blot analysis of TECTA expression in rat testis extract (lane 1), HEPA1-6 whole cell lysates (lane 2) and HEPG2 whole cell lysates (lane 3). TECTA at 239KD was detected using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Related Product Information for anti-TECTA antibody

Rabbit IgG polyclonal antibody for Alpha-tectorin(TECTA) detection.
Background: Alpha-tectorin is a protein that in humans is encoded by the TECTA gene. The tectorial membrane is an extracellular matrix of the inner ear that contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia, and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals. Alpha-tectorin is one of the major noncollagenous components of the tectorial membrane. Mutations in the TECTA gene have been shown to be responsible for autosomal dominant nonsyndromic hearing impairment and a recessive form of sensorineural pre-lingual non-syndromic deafness.

References

1. "Entrez Gene: TECTA tectorin alpha".
2. Hughes DC, Legan PK, Steel KP, Richardson GP (Apr 1998). "Mapping of the alpha-tectorin gene (TECTA) to mouse chromosome 9 and human chromosome 11: a candidate for human autosomal dominant nonsyndromic deafness". Genomics. 48 (1): 46-51.
3. Verhoeven K, Van Laer L, Kirschhofer K, Legan PK, Hughes DC, Schatteman I, Verstreken M, Van Hauwe P, Coucke P, Chen A, Smith RJ, Somers T, Offeciers FE, Van de Heyning P, Richardson GP, Wachtler F, Kimberling WJ, Willems PJ, Govaerts PJ, Van Camp G (May 1998). "Mutations in the human alpha-tectorin gene cause autosomal dominant non-syndromic hearing impairment". Nat Genet. 19 (1): 60-2.

NCBI and Uniprot Product Information

• NCBI GI #: 134268640
• NCBI GeneID: 7007
• NCBI Accession #: NP_005413.2
• NCBI GenBank Nucleotide #: NM_005422.2
• UniProt Accession #: O75443
• NCBI Official Full Name: alpha-tectorin
• NCBI Official Synonym Full Names: tectorin alpha
• NCBI Official Symbol: TECTA
• NCBI Official Synonym Symbols: DFNA8; DFNA12; DFNB21
• NCBI Protein Information: alpha-tectorin
• UniProt Protein Name: Alpha-tectorin
• UniProt Gene Name: TECTA