IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded Human stomach. 1,Antibody was diluted at 1:400 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).)
Rabbit TEK Polyclonal Antibody | anti-TEK antibody
TEK
Gene Names
TEK; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
TEK, Antibody; TEK; CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1; anti-TEK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody detects endogenous levels of TEK.
Purity/Purification
Affinity purification
Form/Format
Liquid in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
1124
Applicable Applications for anti-TEK antibody
ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant protein of human TEK
Conjugation
APC
Cellular Localization
Cell membrane ; Single-pass type I membrane protein. Cell junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton. Secreted . Recruited to cell-cell contacts in quiescent endothelial cells (PubMed:18425120, PubMed:18425119). Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted (PubMed:11806244). Tissue specificity:Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their
progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
Function
Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.,
Function
This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 3 EGF-like domains.,similarity:Contains 3 f ibronectin type-III domains.,tissue specificity:Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney
Preparation and Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycles
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded Human stomach. 1,Antibody was diluted at 1:400 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin- embedded Human stomach. 1,Antibody was diluted at 1:400 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).)
WB (Western Blot)
(Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120kD (Observed)
NCBI Official Full Name
angiopoietin-1 receptor isoform 1
NCBI Official Synonym Full Names
TEK receptor tyrosine kinase
NCBI Official Symbol
TEK
NCBI Official Synonym Symbols
TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
NCBI Protein Information
angiopoietin-1 receptor
UniProt Protein Name
Angiopoietin-1 receptor
UniProt Gene Name
TEK
UniProt Synonym Gene Names
TIE2; VMCM; VMCM1; hTIE2
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Product Notes
The TEK tek (Catalog #AAA268473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TEK reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TEK can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the TEK tek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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