WB (Western Blot)
(Western blot analysis of TF Antibody (C-term) in MCF7 cell line lysates (35ug/lane). TF (arrow) was detected using the purified Pab.)
Rabbit anti-Human TF Polyclonal Antibody | anti-TF antibody
TF Antibody (C-term)
Gene Names
TF; TFQTL1; PRO1557; PRO2086
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TF, Antibody; TF Antibody (C-term); Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF; anti-TF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-654 amino acids from the C-terminal region of human TF.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
627-654
Sequence Length
698
Applicable Applications for anti-TF antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of TF Antibody (C-term) in MCF7 cell line lysates (35ug/lane). TF (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of TF Antibody (C-term) in MCF7 cell line lysates (35ug/lane). TF (arrow) was detected using the purified Pab.)
FCM/FACS (Flow Cytometry)
(TF Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TF Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-TF antibody
TF is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.
Product Categories/Family for anti-TF antibody
References
Mason,A.B., et.al., Biochemistry 32 (20), 5472-5479 (1993)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77064
NCBI Official Full Name
serotransferrin
NCBI Official Synonym Full Names
transferrin
NCBI Official Symbol
TF
NCBI Official Synonym Symbols
TFQTL1; PRO1557; PRO2086
NCBI Protein Information
serotransferrin
UniProt Protein Name
Serotransferrin
UniProt Gene Name
TF
UniProt Synonym Gene Names
Transferrin
UniProt Entry Name
TRFE_HUMAN
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Product Notes
The TF tf (Catalog #AAA287665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 627-654. The TF Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TF can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TF tf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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