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WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using TF3C3 Antibody.)
Rabbit anti-Human, Mouse TF3C3 Polyclonal Antibody | anti-TF3C3 antibody
TF3C3 Antibody
Gene Names
GTF3C3; TFIIIC102; TFIIICgamma; TFiiiC2-102
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TF3C3, Antibody; TF3C3 Antibody; General transcription factor 3C polypeptide 3; general transcription factor IIIC, polypeptide 3 (102kD); general transcription factor IIIC, polypeptide 3, 102kD; GTF3C3; TF3C-gamma; TF3C3_HUMAN; TFIIIC 102 kDa subunit; TFIIIC gamma; TFIIIC102; TFIIIC2 102; TFIIICgamma; Transcription factor IIIC 102 kDa subunit; transcription factor IIIC gamma subunit; Transcription factor IIIC subunit gamma; transcription factor IIIC, 102 kD; anti-TF3C3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TF3C3 antibody detects endogenous levels of total TF3C3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
413
Applicable Applications for anti-TF3C3 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TF3C3
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (92%), Bovine (92%), Horse (86%), Sheep (92%), Rabbit (92%), Dog (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using TF3C3 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using TF3C3 Antibody.)
IF (Immunofluorescence)
(AAA327644 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA327644 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of TF3C3 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of TF3C3 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from 293, using TF3C3 Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using TF3C3 Antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-TF3C3 antibody
Function: Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Subunit Structure: Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1 and TBP.
Subunit Structure: Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1 and TBP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 101 kDa
Predicted: 102 kDa
Predicted: 102 kDa
NCBI Official Full Name
general transcription factor 3C polypeptide 3 isoform 2
NCBI Official Synonym Full Names
general transcription factor IIIC subunit 3
NCBI Official Symbol
GTF3C3
NCBI Official Synonym Symbols
TFIIIC102; TFIIICgamma; TFiiiC2-102
NCBI Protein Information
general transcription factor 3C polypeptide 3
UniProt Protein Name
General transcription factor 3C polypeptide 3
UniProt Gene Name
GTF3C3
UniProt Synonym Gene Names
TFIIIC 102 kDa subunit; TFIIIC102; TF3C-gamma
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Product Notes
The TF3C3 gtf3c3 (Catalog #AAA327644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TF3C3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TF3C3 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the TF3C3 gtf3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TF3C3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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