FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-TGIF/TGIF1 antibody (AAA126354).Overlay histogram showing PC-3 cells stained with AAA126354 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGIF/TGIF1 Antibody (AAA126354, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human TGIF/TGIF1 Polyclonal Antibody | anti-TGIF1 antibody
Anti-TGIF/TGIF1 Antibody Picoband
Gene Names
TGIF1; HPE4; TGIF
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
TGIF/TGIF1, Antibody; Anti-TGIF/TGIF1 Antibody Picoband; anti-TGIF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-TGIF1 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human TGIF/TGIF1 recombinant protein (Position: D26-A401).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-TGIF/TGIF1 antibody (AAA126354).Overlay histogram showing PC-3 cells stained with AAA126354 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGIF/TGIF1 Antibody (AAA126354, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-TGIF/TGIF1 antibody (AAA126354).Overlay histogram showing PC-3 cells stained with AAA126354 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGIF/TGIF1 Antibody (AAA126354, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of TGIF/TGIF1 using anti-TGIF/TGIF1 antibody (AAA126354).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF/TGIF1 antigen affinity purified polyclonal antibody (#AAA126354) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TGIF/TGIF1 at approximately 43 kDa. The expected band size for TGIF/TGIF1 is at 43 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of TGIF/TGIF1 using anti-TGIF/TGIF1 antibody (AAA126354).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF/TGIF1 antigen affinity purified polyclonal antibody (#AAA126354) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TGIF/TGIF1 at approximately 43 kDa. The expected band size for TGIF/TGIF1 is at 43 kDa.)
Related Product Information for anti-TGIF1 antibody
Homeobox protein TGIF1 is a protein that, in humans, is encoded by the TGIF1 gene. The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.
Product Categories/Family for anti-TGIF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,715 Da
NCBI Official Full Name
TGIF, partial
NCBI Official Synonym Full Names
TGFB-induced factor homeobox 1
NCBI Official Symbol
TGIF1
NCBI Official Synonym Symbols
HPE4; TGIF
NCBI Protein Information
homeobox protein TGIF1
UniProt Protein Name
Homeobox protein TGIF1
UniProt Gene Name
TGIF1
UniProt Synonym Gene Names
TGIF
UniProt Entry Name
TGIF1_HUMAN
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Product Notes
The TGIF1 tgif1 (Catalog #AAA126354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TGIF/TGIF1 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TGIF/TGIF1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the TGIF1 tgif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGIF/TGIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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