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ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-THOC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the RHOX5 promoter.)
Rabbit anti-Human THOC1 Polyclonal Antibody | anti-THOC1 antibody
Rabbit anti-human THOC1 polyclonal Antibody
Gene Names
THOC1; P84; HPR1; P84N5
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
THOC1, Antibody; Rabbit anti-human THOC1 polyclonal Antibody; Tho1; Nuclear matrix protein p84; p84N5; hTREX84; THOC1; HPR1; anti-THOC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
657
Applicable Applications for anti-THOC1 antibody
ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human THO complex subunit 1 protein (428-657aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69035
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-THOC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the RHOX5 promoter.)
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-THOC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the RHOX5 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA119500 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA119500 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119500 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119500 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA119500 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA119500 at dilution 1:100)
WB (Western Blot)
(Western blotAll lanes: THOC1 antibody at 3ug/mlLane 1: Jurkats whole cell lysateLane 2: 293T whole cell lysateLane 3: HepG2 whole cell lysateLane 4: A549 whole cell lysateLane 5: Mouse kidney tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 76,44 kDaObserved band size: 76 kDa)
WB (Western Blot)
(Western blotAll lanes: THOC1 antibody at 3ug/mlLane 1: Jurkats whole cell lysateLane 2: 293T whole cell lysateLane 3: HepG2 whole cell lysateLane 4: A549 whole cell lysateLane 5: Mouse kidney tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 76,44 kDaObserved band size: 76 kDa)
Related Product Information for anti-THOC1 antibody
Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,359 Da
NCBI Official Full Name
THO complex subunit 1
NCBI Official Synonym Full Names
THO complex 1
NCBI Official Symbol
THOC1
NCBI Official Synonym Symbols
P84; HPR1; P84N5
NCBI Protein Information
THO complex subunit 1
UniProt Protein Name
THO complex subunit 1
UniProt Gene Name
THOC1
UniProt Synonym Gene Names
HPR1; Tho1; p84N5
UniProt Entry Name
THOC1_HUMAN
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Product Notes
The THOC1 thoc1 (Catalog #AAA119500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human THOC1 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's THOC1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the THOC1 thoc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THOC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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