WB (Western Blot)
(Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).)
Rabbit anti-Arabidopsis Thr449 S6 kinase 1 Polyclonal Antibody | anti-S6K1 antibody
Anti-Phospho-Thr449 S6 kinase 1 (S6K1)
Gene Names
PK1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1
Reactivity
Arabidopsis
Applications
Western Blot
Purity
Antigen Affinity Purified from the Pooled Serum
Synonyms
Thr449 S6 kinase 1, Antibody; Anti-Phospho-Thr449 S6 kinase 1 (S6K1); S6 Kinase 1 (Thr449); anti-S6K1 antibody
Host
Rabbit
Reactivity
Arabidopsis
Clonality
Polyclonal
Specificity
Specific for the ~53k S6K1 protein phosphorylated at Thr449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
Purity/Purification
Antigen Affinity Purified from the Pooled Serum
Form/Format
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. (Adequate amount of material to conduct 10 mini Western blots)
Sequence Length
465
Applicable Applications for anti-S6K1 antibody
WB (Western Blot)
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 of Arabidopsis S6K1, conjugated to Keyhole Limpet Hemocyanin (KLH).
Preparation and Storage
For long term storage -20°C is recommended. Stable for at least one year.
WB (Western Blot)
(Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).)
WB (Western Blot)
(Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).)
Related Product Information for anti-S6K1 antibody
Affinity purified rabbit polyclonal antibody
Description: Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at Thr-389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 Thr-449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at Thr-449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 Thr-449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
Description: Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at Thr-389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 Thr-449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at Thr-449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 Thr-449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53 kDa
NCBI Official Full Name
protein-serine kinase 1
NCBI Official Symbol
PK1
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1
NCBI Protein Information
protein-serine kinase 1
UniProt Protein Name
Serine/threonine-protein kinase AtPK1/AtPK6
UniProt Gene Name
ATPK1
UniProt Synonym Gene Names
ATPK6; S6K1
UniProt Entry Name
KPK1_ARATH
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Product Notes
The S6K1 atpk1 (Catalog #AAA72825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Thr449 S6 kinase 1 (S6K1) reacts with Arabidopsis and may cross-react with other species as described in the data sheet. AAA Biotech's Thr449 S6 kinase 1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the S6K1 atpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thr449 S6 kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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