IF (Immunofluorescence)
(Immunofluorescence staining of TPO in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPO polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)
Rabbit anti-Human Thyroid peroxidase Polyclonal Antibody | anti-TPO antibody
Anti-Thyroid peroxidase Antibody, Rabbit Polyclonal
Gene Names
TPO; MSA; TPX; TDH2A
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Synonyms
Thyroid peroxidase, Antibody; Anti-Thyroid peroxidase Antibody, Rabbit Polyclonal; Thyroid peroxidase/TPO Antibody, Rabbit PAb, Antigen Affinity Purified; thyroid peroxidase; Anti-MSA Antibody; Anti-TDH2A Antibody; Anti-TPX Antibody; anti-TPO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Thyroid peroxidase/TPO
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TPO antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Thyroid peroxidase/TPO Protein ( )
Preparation
Produced in rabbits immunized with purified, recombinant Human Thyroid peroxidase/TPO ; ( ) (rh Thyroid peroxidase/TPO). Thyroid peroxidase/TPO specific IgG was purified by Human Thyroid peroxidase/TPO affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity.
Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Avoid repeated freeze-thaw cycles.
Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of TPO in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPO polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of TPO in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TPO polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TPO in human thyroid with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TPO in human thyroid with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
WB (Western Blot)
(Anti-TPO rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:103 kDaObserved band size:123 kDa)
WB (Western Blot)
(Anti-TPO rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:103 kDaObserved band size:123 kDa)
Related Product Information for anti-TPO antibody
Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.
References
McLachlan SM, et al. (2000) Autoimmune response to the thyroid in humans: thyroid peroxidase--the common autoantigenic denominator. Int Rev Immunol. 19(6):587-618.Nagasaka A, et al. (1976) Effect of antithyroid agents 6-propyl-2-thiouracil and 1-mehtyl-2-mercaptoimidazole on human thyroid iodine peroxidase. J Clin Endocrinol Metab. 43(1): 152-8.Kimura S, et al. (1987) Human thyroid peroxidase: complete cDNA and protein sequence, chromosome mapping, and identification of two alternately spliced mRNAs. Proc Natl Acad. 84(16):5555-9.Ruf J, et al. (2006) Structural and functional aspects of thyroid peroxidase. Arch Biochem Biophys. 445 (2):269-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
thyroid peroxidase isoform a
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
TPO
NCBI Official Synonym Symbols
MSA; TPX; TDH2A
NCBI Protein Information
thyroid peroxidase; thyroperoxidase; thyroid microsomal antigen
UniProt Protein Name
Thyroid peroxidase
UniProt Gene Name
TPO
UniProt Synonym Gene Names
TPO
UniProt Entry Name
PERT_HUMAN
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Product Notes
The TPO tpo (Catalog #AAA255653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Thyroid peroxidase Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thyroid peroxidase can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TPO tpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroid peroxidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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