WB (Western Blot)
(Anti-TIE2 Picoband antibody, -2.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD)
anti-Human TIE2 Polyclonal Antibody | anti-TIE2 antibody
Anti-TIE2 Antibody
Gene Names
TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
TIE2, Antibody; Anti-TIE2 Antibody; Angiopoietin-1 receptor; Angiopoietin 1 receptor; CD202b; CD202b antigen; Endothelial tyrosine kinase; Endothelium specific receptor tyrosine kinase 2; hTIE 2; hTIE2; Hyk; p140 TEK; Soluble TIE2 variant 1; Soluble TIE2 variant 2; TEK; tek tyrosine kinase; TEK tyrosine kinase endothelial; tek tyrosine kinase, endothelial; TIE 2; TIE2_HUMAN; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains 2; Tyrosine protein kinase receptor TEK; Tyrosine protein kinase receptor TIE 2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; Venous malformations multiple cutaneous and mucosal; VMCM 1; VMCM; VMCM1; TEK tyrosine kinase, endothelial; anti-TIE2 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1124
Applicable Applications for anti-TIE2 antibody
WB (Western Blot)
Immunogen
E Coli-derived human TIE2 recombinant protein (Position: Q641-I830). Human TIE2 shares 91% amino acid (aa) sequence identity with mouse TIE2.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-TIE2 Picoband antibody, -2.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD)
WB (Western Blot)
(Anti-TIE2 Picoband antibody, -2.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD)
WB (Western Blot)
(Anti-TIE2 Picoband antibody, -1.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: Recombinant Human TIE2 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)
WB (Western Blot)
(Anti-TIE2 Picoband antibody, -1.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: Recombinant Human TIE2 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)
Related Product Information for anti-TIE2 antibody
Description: Rabbit IgG polyclonal antibody for Angiopoietin-1 receptor(TEK) detection. Tested with WB in Human.
Background: TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
Background: TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
References
1. Arai, F., Hirao, A., Ohmura, M., Sato, H., Matsuoka, S., Takubo, K., Ito, K., Koh, G. Y., Suda, T. Tie2/Angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell 118: 149-161, 2004. 2. Ramsden, J. D., Cocks, H. C., Shams, M., Nijjar, S., Watkinson, J. C., Sheppard, M. C., Ahmed, A., Eggo, M. C. Tie-2 is expressed on thyroid follicular cells, is increased in goiter, and is regulated by thyrotropin through cyclic adenosine 3-prime,5-prime-monophosphate. J. Clin. Endocr. Metab. 86: 2709-2716, 2001.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,141 Da
NCBI Official Full Name
angiopoietin-1 receptor isoform 1
NCBI Official Synonym Full Names
TEK receptor tyrosine kinase
NCBI Official Symbol
TEK
NCBI Official Synonym Symbols
TIE2; VMCM; TIE-2; VMCM1; CD202B
NCBI Protein Information
angiopoietin-1 receptor
UniProt Protein Name
Angiopoietin-1 receptor
UniProt Gene Name
TEK
UniProt Synonym Gene Names
TIE2; VMCM; VMCM1; hTIE2
UniProt Entry Name
TIE2_HUMAN
Similar Products
Product Notes
The TIE2 tek (Catalog #AAA46301) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIE2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIE2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TIE2 tek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.