WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using Tim23 antibody.)
Rabbit Tim23 Polyclonal Antibody | anti-Tim23 antibody
Tim23 Antibody
Gene Names
TIMM23; TIM23
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Tim23, Antibody; Tim23 Antibody; MGC22767; MGC93478; Mitochondrial import inner membrane translocase subunit Tim23; Predicted protein of HQ1197; PRO1197; TIM23; TIMM 23; TIMM23; Translocase of inner mitochondrial membrane 23 (yeast) homolog; Translocase of inner mitochondrial membrane 23 homolog (yeast); Translocase of inner mitochondrial membrane 23 homolog B; Translocation of mitochondrial precursor proteins; anti-Tim23 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Tim23 antibody detects endogenous levels of Tim23.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
209
Applicable Applications for anti-Tim23 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Tim23.
Subcellular Location
Mitochondrion Inner Membrane; Multipass Membrane Protein.
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Xenopus
Similarity
Zebrafish (92%), Bovine (100%), Horse (100%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using Tim23 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using Tim23 antibody.)
IHC (Immunohistochemistry)
(AAA322879 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322879 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-Tim23 antibody
Function: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Subunit Structure: Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM50. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15.
Similarity: Belongs to the Tim17/Tim22/Tim23 family.
Subunit Structure: Component of the TIM23 complex at least composed of TIMM23, TIMM17 (TIMM17A or TIMM17B) and TIMM50; within this complex, directly interacts with TIMM50. The complex interacts with the TIMM44 component of the PAM complex and with DNAJC15.
Similarity: Belongs to the Tim17/Tim22/Tim23 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
mitochondrial import inner membrane translocase subunit Tim23
NCBI Official Synonym Full Names
translocase of inner mitochondrial membrane 23
NCBI Official Symbol
TIMM23
NCBI Official Synonym Symbols
TIM23
NCBI Protein Information
mitochondrial import inner membrane translocase subunit Tim23
UniProt Protein Name
Mitochondrial import inner membrane translocase subunit Tim23
UniProt Gene Name
TIMM23
UniProt Synonym Gene Names
TIM23
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Product Notes
The Tim23 timm23 (Catalog #AAA322879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tim23 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tim23 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Tim23 timm23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tim23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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