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product-image-AAA322964_WB11.jpg WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using TIP47 antibody.)

Rabbit anti-Human, Mouse TIP47 Polyclonal Antibody | anti-TIP47 antibody

TIP47 Antibody

Gene Names
PLIN3; PP17; TIP47; M6PRBP1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TIP47, Antibody; TIP47 Antibody; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; Mannose-6-phosphate receptor-binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin-3; Perilipin3; Placental protein 17; PLIN3; PLIN3_HUMAN; pp17; Tail interacting protein 47 kD; TIP47; anti-TIP47 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TIP47 antibody detects endogenous levels of TIP47.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
433
Applicable Applications for anti-TIP47 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TIP47.
Subcellular Location
Cytoplasm. Endosome Membrane. Lipid Droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts of HeLa cells, using TIP47 antibody.)

product-image-AAA322964_WB11.jpg WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using TIP47 antibody.)

WB (Western Blot)

(Western blot analysis on HeLa cells lysate using TIP47 Antibody)

product-image-AAA322964_WB13.jpg WB (Western Blot) (Western blot analysis on HeLa cells lysate using TIP47 Antibody)

IHC (Immunohistochemistry)

(AAA322964 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

product-image-AAA322964_IHC15.jpg IHC (Immunohistochemistry) (AAA322964 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-TIP47 antibody
Function: Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Subunit Structure: Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C).
Similarity: Belongs to the perilipin family.
References
Guo L, Tian M, Feng R, Zhang G, Zhang R, Li X, Liu Z, He X, Sun JZ, Yu X; Journal: ACS Appl Mater Interfaces. Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 47 kDa
Predicted: 48 kDa
NCBI Official Full Name
perilipin-3 isoform 2
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
NCBI Protein Information
perilipin-3
UniProt Protein Name
Perilipin-3
UniProt Gene Name
PLIN3
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17

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Product Notes

The TIP47 plin3 (Catalog #AAA322964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIP47 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIP47 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TIP47 plin3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIP47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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