Rabbit TK1 Polyclonal Antibody | anti-TK1 antibody

TK1 Antibody

Cat. Num. AAA303686

• Gene Names: TK1; TK2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TK1, Antibody; TK1 Antibody; TK2; anti-TK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TK1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 234

• Applicable Applications for anti-TK1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TK1.
• Target Name: TK1
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using TK1 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using TK1 antibody.)

Related Product Information for anti-TK1 antibody

Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell. There are two known thymidine kinases, cytoplasmic thymidine kinase 1 (TK1) and mitochondrial thymidine kinase 2 (TK2) (1,2). Unlike TK2, which is not modulated by the cell cycle, TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division (3,4). Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive (5). These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response (6). Genotoxic stress promotes increased TK1 expression and kinase activity resulting in reduced cellular apoptosis and enhanced DNA repair efficiency (6). More importantly, numerous studies show that TK1 expression and activity are upregulated during neoplasia and disease progression in humans, and increased serum levels of TK1 correlate with poor prognosis and decreased survival in patients with various types of advanced tumors (7-12).

Product Categories/Family for anti-TK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 164698438
• NCBI GeneID: 7083
• NCBI Accession #: NP_003249.3
• NCBI GenBank Nucleotide #: NM_003258.4
• UniProt Accession #: P04183; Q969V0; Q9UMG9; B2RC58
• Molecular Weight: 25,469 Da
• NCBI Official Full Name: thymidine kinase, cytosolic
• NCBI Official Synonym Full Names: thymidine kinase 1, soluble
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic
• UniProt Protein Name: Thymidine kinase, cytosolic
• UniProt Gene Name: TK1
• UniProt Entry Name: KITH_HUMAN