Rabbit TLR4 Polyclonal Antibody | anti-TLR4 antibody

TLR4 Antibody (Center)

Cat. Num. AAA286138

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Reactivity: Human; Predicted Reactivity: Bovine, Hamster, Horse, Pig
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TLR4, Antibody; TLR4 Antibody (Center); Toll-like receptor 4; hToll; CD284; TLR4; anti-TLR4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Hamster, Horse, Pig
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-698 amino acids from the Central region of human TLR4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 669-698
• Sequence Length: 639

• Applicable Applications for anti-TLR4 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Funtion: Cooperates with LY96 and CD14 to mediatethe innate immune response to bacteriallipopolysaccharide (LPS) (PubMed: ). Actsvia MYD88, TIRAP and TRAF6, leading toNF-kappa-B activation, cytokine secretionand the inflammatory response (PubMed:9237759,PubMed: ,PubMed: ). Alsoinvolved in LPS-independent inflammatoryresponses triggered by free fatty acids,such as palmitate, and Ni(2+). Responsestriggered by Ni(2+) require non- conservedhistidines and are, therefore,species-specific (PubMed: ). BothM.tuberculosis HSP70 (dnaK) and HSP65(groEL-2) act via this protein to stimulateNF-kappa-B expression (PubMed: ). Incomplex with TLR6, promotes sterileinflammation in monocytes/macrophages inresponse to oxidized low-density lipoprotein(oxLDL) or amyloid-beta 42. In this context,the initial signal is provided by oxLDL- oramyloid-beta 42-binding to CD36. Thisevent induces the formation of aheterodimer of TLR4 and TLR6, which israpidly internalized and triggersinflammatory response, leading to theNF-kappa-B-dependent production ofCXCL1, CXCL2 and CCL9 cytokines, viaMYD88 signaling pathway, and CCL5cytokine, via TICAM1 signaling pathway, aswell as IL1B secretion. Bindselectronegative LDL (LDL(-)) and mediatesthe cytokine release induced by LDL(-)(PubMed: ).Stimulation of monocytes in vitro withM.tuberculosis PstS1 induces p38 MAPK andERK1/2 activation primarily via TLR2, butalso partially via this receptor (PubMed: ).
• Cellular Location: Cell membrane; Single-pass type Imembrane protein. Early endosome. Cellprojection, ruffle{ECO:0000250|UniProtKB:Q9QUK6}.Note=Upon complex formation with CD36and TLR6, internalized throughdynamin-dependent endocytosis(PubMed:20037584). Colocalizes withRFTN1 at cell membrane and then togetherwith RFTN1 moves to endosomes, uponlipopolysaccharide stimulation.
• Tissue Location: Highly expressed in placenta, spleen andperipheral blood leukocytes(PubMed:9435236, PubMed:9237759).Detected in monocytes, macrophages,dendritic cells and several types of T-cells(PubMed:9237759, PubMed:27022195).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(TLR4 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of TLR4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). TLR4 (arrow) was detected using the purified Pab.)

Related Product Information for anti-TLR4 antibody

TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.

Product Categories/Family for anti-TLR4 antibody

Cardiovascular; Immunology; Microbiology; Signal Transduction

References

Sam-Agudu, N.A., et al. Am. J. Trop. Med. Hyg. 82(4):548-555(2010)
# Palomino-Morales, R.J., et al. Arthritis Res. Ther. 12 (2), R51 (2010)
# Rigoli, L., et al. Anticancer Res. 30(2):513-517(2010)
# da Silva Correia, J., et al. J. Biol. Chem. 277(3):1845-1854(2002)
# Shimazu, R., et al. J. Exp. Med. 189(11):1777-1782(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 4507533
• NCBI GeneID: 7099
• NCBI Accession #: NP_003257.1; NP_612564.1; NP_612567.1
• NCBI GenBank Nucleotide #: NM_003266.3
• UniProt Accession #: O00206; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53
• NCBI Official Full Name: toll-like receptor 4 isoform C
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• UniProt Gene Name: TLR4
• UniProt Entry Name: TLR4_HUMAN