FCM/FACS (Flow Cytometry)
(TLR9 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human TLR9 Polyclonal Antibody | anti-TLR9 antibody
TLR9 Antibody (C-term)
Gene Names
TLR9; CD289
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TLR9, Antibody; TLR9 Antibody (C-term); Toll-like receptor 9; CD289; TLR9; anti-TLR9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TLR9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 842-870 amino acids from the C-terminal region of human TLR9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.31 (varies by lot)
Sequence Positions
842-870
Sequence Length
1,017
Applicable Applications for anti-TLR9 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TLR9 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TLR9 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(TLR9 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).)
WB (Western Blot)
(TLR9 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).)
Related Product Information for anti-TLR9 antibody
The protein encoded by this gene is a member of the
Toll-like receptor (TLR) family which plays a fundamental role in
pathogen recognition and activation of innate immunity. TLRs are
highly conserved from Drosophila to humans and share structural and
functional similarities. They recognize pathogen-associated
molecular patterns (PAMPs) that are expressed on infectious agents,
and mediate the production of cytokines necessary for the
development of effective immunity. The various TLRs exhibit
different patterns of expression. This gene is preferentially
expressed in immune cell rich tissues, such as spleen, lymph node,
bone marrow and peripheral blood leukocytes. Studies in mice and
human indicate that this receptor mediates cellular response to
unmethylated CpG dinucleotides in bacterial DNA to mount an innate
immune response.
Toll-like receptor (TLR) family which plays a fundamental role in
pathogen recognition and activation of innate immunity. TLRs are
highly conserved from Drosophila to humans and share structural and
functional similarities. They recognize pathogen-associated
molecular patterns (PAMPs) that are expressed on infectious agents,
and mediate the production of cytokines necessary for the
development of effective immunity. The various TLRs exhibit
different patterns of expression. This gene is preferentially
expressed in immune cell rich tissues, such as spleen, lymph node,
bone marrow and peripheral blood leukocytes. Studies in mice and
human indicate that this receptor mediates cellular response to
unmethylated CpG dinucleotides in bacterial DNA to mount an innate
immune response.
Product Categories/Family for anti-TLR9 antibody
References
Engin, A., et al. Microbes Infect. 12 (12-13), 1071-1078 (2010) :
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Veltkamp, M., et al. Clin. Exp. Immunol. 162(1):68-74(2010)
Fiola, S., et al. J. Immunol. 185(6):3620-3631(2010)
Selvaraj, P., et al. Tuberculosis (Edinb) 90(5):306-310(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Veltkamp, M., et al. Clin. Exp. Immunol. 162(1):68-74(2010)
Fiola, S., et al. J. Immunol. 185(6):3620-3631(2010)
Selvaraj, P., et al. Tuberculosis (Edinb) 90(5):306-310(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115860
NCBI Official Full Name
toll-like receptor 9
NCBI Official Synonym Full Names
toll-like receptor 9
NCBI Official Symbol
TLR9
NCBI Official Synonym Symbols
CD289
NCBI Protein Information
toll-like receptor 9
UniProt Protein Name
Toll-like receptor 9
UniProt Gene Name
TLR9
UniProt Entry Name
TLR9_HUMAN
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Product Notes
The TLR9 tlr9 (Catalog #AAA286642) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 842-870. The TLR9 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLR9 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TLR9 tlr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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