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IP (Immunoprecipitation)
(TMED2 was immunoprecipitated using:Lane A:0.5 mg RAJI Whole Cell Lysate4 uL anti-TMED2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMED2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
Rabbit anti-Human TMED2 Polyclonal Antibody | anti-TMED2 antibody
Anti-TMED2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TMED2, Antibody; Anti-TMED2 Antibody, Rabbit Polyclonal; TMED2 Antibody, Rabbit PAb, Antigen Affinity Purified; transmembrane emp24 domain trafficking protein 2; Anti-p24 Antibody; Anti-P24A Antibody; Anti-RNP24 Antibody; anti-TMED2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMED2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TMED2 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human TMED2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TMED2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(TMED2 was immunoprecipitated using:Lane A:0.5 mg RAJI Whole Cell Lysate4 uL anti-TMED2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMED2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
IP (Immunoprecipitation)
(TMED2 was immunoprecipitated using:Lane A:0.5 mg RAJI Whole Cell Lysate4 uL anti-TMED2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMED2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :23 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human TMED2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human TMED2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMED2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMED2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-TMED2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Raji Whole Cell LysateLane D: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-TMED2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Raji Whole Cell LysateLane D: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-TMED2 antibody
TMED2, a member of the transmembrane emp24 domain (TMED) family, is required for the transport of cargo proteins between the ER and Golgi. Tmed2, the sole member of the vertebrate Tmed beta subfamily, exhibits tissue and temporal specific patterns of expression in embryos and developing placenta but is ubiquitously expressed in all adult organs. TMED2 is involved in the morphogenesis of the mouse embryo and placenta. TMED2 is important for the normal morphogenesis of mouse embryos and their associated placenta, and in fact, Tmed2 homozygous mutant embryos arrest at mid-gestation due to a failure of placental labyrinth layer formation. TMED2 is oncogenic and a potential target for epithelial ovarian cancer therapy.
References
Shi-Peng G, et al. (2017) Tmed2 promotes epithelial ovarian cancer growth. Oncotarget 8 (55): 94151-94165.Hou W, et al. (2018) Tmed2/emp24 is required in both the chorion and the allantois for placental labyrinth layer development. Dev Biol 444 (1): 20-32.Hou W, et al. (2017) Non-alcoholic fatty liver disease in mice with heterozygous mutation in tmed2. PLoS One 12 (8): e0182995.
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Product Notes
The TMED2 (Catalog #AAA256083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMED2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMED2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TMED2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMED2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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