Rabbit anti-Human, mouse TMEM66 Polyclonal Antibody | anti-SARAF antibody

TMEM66 Antibody (N-term)

Cat. Num. AAA285126

• Gene Names: SARAF; XTP3; FOAP-7; TMEM66; HSPC035
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: TMEM66, Antibody; TMEM66 Antibody (N-term); Store-operated calcium entry-associated regulatory factor; SARAF; SOCE-associated regulatory factor; HBV X-transactivated gene 3 protein; HBV XAg-transactivated protein 3; Protein FOAP-7; Transmembrane protein 66; TMEM66; XTP3; anti-SARAF antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This TMEM66 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TMEM66.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 33-62
• Sequence Length: 167

• Applicable Applications for anti-SARAF antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)- dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.
• Cellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca(2+) elevation.
• Tissue Location: Highly expressed in macrophages. {ECO:0000269|Ref.2}
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(TMEM66 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(TMEM66 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)

WB (Western Blot)

(TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)

Related Product Information for anti-SARAF antibody

TMEM66 is a multi-pass membrane protein which belongs to the TMEM66 family. The exact function of TMEM66 remains unknown.

Product Categories/Family for anti-SARAF antibody

Cell Biology

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 42476193
• NCBI GeneID: 51669
• NCBI Accession #: NP_057211.4
• NCBI GenBank Nucleotide #: NM_016127.5
• UniProt Accession #: Q96BY9; Q53HE8; Q9UNZ3; Q9Y683; B3KQQ4; B7Z9J1; D3DSU7; H9MHJ8; H9MHJ9
• Molecular Weight: Calculated MW: 36975
• NCBI Official Full Name: store-operated calcium entry-associated regulatory factor isoform 1
• NCBI Official Synonym Full Names: store-operated calcium entry-associated regulatory factor
• NCBI Official Symbol: SARAF
• NCBI Official Synonym Symbols: XTP3; FOAP-7; TMEM66; HSPC035
• NCBI Protein Information: store-operated calcium entry-associated regulatory factor
• UniProt Protein Name: Store-operated calcium entry-associated regulatory factor
• UniProt Gene Name: SARAF
• UniProt Synonym Gene Names: TMEM66; XTP3; SARAF; SOCE-associated regulatory factor
• UniProt Entry Name: SARAF_HUMAN