WB (Western Blot)
(Anti-TMPO rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:57 kDa)
Rabbit anti-Human TMPO Polyclonal Antibody | anti-TMPO antibody
Anti-TMPO Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TMPO, Antibody; Anti-TMPO Antibody, Rabbit Polyclonal; thymopoietin; Anti-CMD1T Antibody; Anti-LAP2 Antibody; Anti-LEMD4 Antibody; Anti-PRO0868 Antibody; Anti-TP Antibody; anti-TMPO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMPO
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TMPO antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E coli-derived Human TMPO fragment
Preparation
Produced in rabbits immunized with E coli-derived Human TMPO fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-TMPO rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:57 kDa)
WB (Western Blot)
(Anti-TMPO rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:51 kDaObserved band size:57 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMPO in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMPO in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IP (Immunoprecipitation)
(TMPO was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg 293T Whole Cell Lysate4 uL anti-TMPO rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMPO rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size :56 kDa)
IP (Immunoprecipitation)
(TMPO was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg 293T Whole Cell Lysate4 uL anti-TMPO rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TMPO rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size :56 kDa)
Related Product Information for anti-TMPO antibody
Thymopoietin is a polypeptide hormone of the thymus consisting of 49 amino acids. The pentapeptide thymopentin (TP-5) Arg-Lys-Asp-Val-Tyr, corresponding to amino acids 32-36 of thymopoietin, appears to represent the active site of thymopoietin in that it has all the biological activities of the native hormone. Thymopoietin is secreted by epithelial cells of the thymus and is pleiotropic in action, affecting neuromuscular transmission, induction of early T cell differentiation and immune regulation. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells. Thymopoietin and thymopentin have the biological characteristics of being immune normalizing in a number of animal model systems of immune dysbalance. These include immune dysbalances induced by thymectomy or the thymic involution associated with aging or by other procedures in thymus-intact animals. The normalizing action of thymopentin, whether the immune dysbalance be in the direction of hyper- or hyporesponsiveness, points to its potential utility in human diseases characterized by immune dysbalance.
References
Zhang L, et al. (2016) Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells. World J Surg Oncol 14 (1): 267.
Marrero-Rodríguez D, et al. (2015) Thymopoietin beta and gamma isoforms as a potential diagnostic molecular marker for breast cancer: Preliminary data. Pathol Oncol Res 21 (4): 1045-1050.
Goldstein G, et al. (1985) Thymopoietin to thymopentin: Experimental studies. Surv Immunol Res 4 Suppl 1 1-10.
Marrero-Rodríguez D, et al. (2015) Thymopoietin beta and gamma isoforms as a potential diagnostic molecular marker for breast cancer: Preliminary data. Pathol Oncol Res 21 (4): 1045-1050.
Goldstein G, et al. (1985) Thymopoietin to thymopentin: Experimental studies. Surv Immunol Res 4 Suppl 1 1-10.
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Product Notes
The TMPO (Catalog #AAA259012) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMPO Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMPO can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TMPO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMPO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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