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product-image-AAA133456_AD10.jpg Knockout Validation (Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: TRAF6 knockout A431 cell lysate; Predicted MW: 60kd Observed MW: 60kd Primary Ab: 5ug/ml Rabbit Anti-Human TRAF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Rabbit anti-Human, Mouse TNF Receptor Associated Factor 6 (TRAF6) Polyclonal Antibody | anti-TRAF6 antibody

Polyclonal Antibody to TNF Receptor Associated Factor 6 (TRAF6)

Gene Names
TRAF6; RNF85; MGC:3310
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
TNF Receptor Associated Factor 6 (TRAF6), Antibody; Polyclonal Antibody to TNF Receptor Associated Factor 6 (TRAF6); anti-TRAF6 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TRAF6. It has been selected for its ability to recognize TRAF6 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AHCEFALM DCPQCQRPFQ KFHINIHILK DCPRRQVSCD NCAASMAFED KEIHDQNCPL ANVICEYCNT ILIREQMPNH YDLDCPTAPI PCTFSTFGCH EKMQRNHLAR HLQENTQSHM RMLAQAVHSL SVIPDSGYIS EVRNFQETIH QLEGRLVRQD HQIRELTAKM ETQSMYVSEL KRTIRTLEDK VAEIEAQQCN GIYIWKIGNF GMHLKCQEEE KPVVIHSPGF YTGKPGYKLC MRLHLQLPTA QRCANYISLF VHTMQGEYDS HLP
Sequence Length
522
Applicable Applications for anti-TRAF6 antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant TRAF6 (Ala153~Pro423) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Knockout Validation

(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: TRAF6 knockout A431 cell lysate; Predicted MW: 60kd Observed MW: 60kd Primary Ab: 5ug/ml Rabbit Anti-Human TRAF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

product-image-AAA133456_AD10.jpg Knockout Validation (Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: TRAF6 knockout A431 cell lysate; Predicted MW: 60kd Observed MW: 60kd Primary Ab: 5ug/ml Rabbit Anti-Human TRAF6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemisry)

(DAB staining on IHC-P. Samples: Human Tissue))

product-image-AAA133456_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Human Tissue))

WB (Western Blot)

(Western Blot: Sample: Recombinant TRAF6, Human.)

product-image-AAA133456_WB13.jpg WB (Western Blot) (Western Blot: Sample: Recombinant TRAF6, Human.)

WB (Western Blot)

(Western Blot: Sample: Human A431 Cells.)

product-image-AAA133456_WB15.jpg WB (Western Blot) (Western Blot: Sample: Human A431 Cells.)
Product Categories/Family for anti-TRAF6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,573 Da
NCBI Official Full Name
TNF receptor-associated factor 6
NCBI Official Synonym Full Names
TNF receptor associated factor 6
NCBI Official Symbol
TRAF6
NCBI Official Synonym Symbols
RNF85; MGC:3310
NCBI Protein Information
TNF receptor-associated factor 6
UniProt Protein Name
TNF receptor-associated factor 6
UniProt Gene Name
TRAF6
UniProt Synonym Gene Names
RNF85

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Product Notes

The TRAF6 traf6 (Catalog #AAA133456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to TNF Receptor Associated Factor 6 (TRAF6) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNF Receptor Associated Factor 6 (TRAF6) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the TRAF6 traf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AHC EFALM DCPQCQRPFQ KFHINIHILK DCPRRQVSCD NCAASMAFED KEIHDQNCPL ANVICEYCNT ILIREQMPNH YDLDCPTAPI PCTFSTFGCH EKMQRNHLAR HLQENTQSHM RMLAQAVHSL SVIPDSGYIS EVRNFQETIH QLEGRLVRQD HQIRELTAKM ETQSMYVSEL KRTIRTLEDK VAEIEAQQCN GIYIWKIGNF GMHLKCQEEE KPVVIHSPGF YTGKPGYKLC MRLHLQLPTA QRCANYISLF VHTMQGEYDS HLP. It is sometimes possible for the material contained within the vial of "TNF Receptor Associated Factor 6 (TRAF6), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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