WB (Western Blot)
(Western blot analysis of extracts of various celllines,using TNFA antibody.)
Rabbit TNFA Polyclonal Antibody | anti-TNFA antibody
TNFA Antibody
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Sheep, Dog
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TNFA, Antibody; TNFA Antibody; APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form; anti-TNFA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Sheep, Dog
Predicted: Pig, Bovine, Sheep, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
TNFA Antibody detects endogenous levels of total TNFA.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; PBS, pH 7.4,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-TNFA antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TNFA.
Subcellular Location
Plasma Membrane; Extracellular Region or Secreted; Extracellular Region or Secreted; Extracellular Region or Secreted
WB (Western Blot)
(Western blot analysis of extracts of various celllines,using TNFA antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various celllines,using TNFA antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using TNFA antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using TNFA antibody.)
IF (Immunofluorescence)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody , diluted at 1/600, was used as secondary antibod)
IF (Immunofluorescence)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody , diluted at 1/600, was used as secondary antibod)
Related Product Information for anti-TNFA antibody
Description: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).
Post Translational Modifications: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Subunit Structure: Homotrimer. Interacts with SPPL2B.
Similarity: Belongs to the tumor necrosis factor family.
Post Translational Modifications: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Subunit Structure: Homotrimer. Interacts with SPPL2B.
Similarity: Belongs to the tumor necrosis factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 17/30-35 kDa
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The TNFA tnf (Catalog #AAA328228) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFA Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Sheep, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TNFA can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TNFA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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