WB (Western Blot)
(Anti-TNFRSF1B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:45 kDa)
Rabbit anti-Mouse TNFR2/TNFRSF1B Polyclonal Antibody | anti-TNFRSF1B antibody
Anti-TNFR2/TNFRSF1B Antibody, Rabbit Polyclonal
Gene Names
Tnfrsf1b; p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TNFR2/TNFRSF1B, Antibody; Anti-TNFR2/TNFRSF1B Antibody, Rabbit Polyclonal; TNFR2/CD120b/TNFRSF1B Antibody, Rabbit PAb, Antigen Affinity Purified; tumor necrosis factor receptor superfamily, member 1b; Anti-CD120b Antibody; Anti-p75 Antibody; Anti-TNF-alphaR2 Antibody; Anti-TNF-R-II Antibody; Anti-TNF-R2 Antibody; Anti-TNF-R75 Antibody; Anti-TNFalpha-R2 Antibody; Anti-TNFBR Antibody; Anti-Tnfr-1 Antibody; Anti-Tnfr2 Antibody; Anti-TNFR80 Antibody; Anti-TNFRII Antibody; anti-TNFRSF1B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse TNFR2/CD120b/TNFRSF1B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNFRSF1B antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse TNFR2/CD120b/TNFRSF1B protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse TNFR2/CD120b/TNFRSF1B (rM TNFR2/CD120b/TNFRSF1B). TNFR2/CD120b/TNFRSF1B specific IgG was purified by Mouse TNFR2/CD120b/TNFRSF1B affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-TNFRSF1B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:45 kDa)
WB (Western Blot)
(Anti-TNFRSF1B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:45 kDa)
IP (Immunoprecipitation)
(Mouse TNFRSF1B was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-Mouse TNFRSF1B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse TNFRSF1B rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 45 kDa)
IP (Immunoprecipitation)
(Mouse TNFRSF1B was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-Mouse TNFRSF1B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse TNFRSF1B rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 45 kDa)
Related Product Information for anti-TNFRSF1B antibody
Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Product Categories/Family for anti-TNFRSF1B antibody
References
Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,320 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 1B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1b
NCBI Official Symbol
Tnfrsf1b
NCBI Official Synonym Symbols
p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1B; TNF receptor beta chain; TNF-RII; TNFR-II; p75 TNFR; p80 TNF-alpha receptor; tumor necrosis factor receptor 2; tumor necrosis factor receptor type II
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1B
UniProt Gene Name
Tnfrsf1b
UniProt Synonym Gene Names
Tnfr-2; Tnfr2; TNF-R2; TNF-RII; TNFR-II
UniProt Entry Name
TNR1B_MOUSE
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Product Notes
The TNFRSF1B tnfrsf1b (Catalog #AAA257092) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNFR2/TNFRSF1B Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNFR2/TNFRSF1B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TNFRSF1B tnfrsf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFR2/TNFRSF1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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