WB (Western Blot)
(Western blot analysis on HuvEc, Jurkat and 293 cell lysate using TNFSF12 Antibody.The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse TNFSF12 Polyclonal Antibody | anti-TNFSF12 antibody
TNFSF12 Antibody
Gene Names
TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TNFSF12, Antibody; TNFSF12 Antibody; APO 3 ligand; APO 3L; APO3 ligand; APO3/DR3 ligand; APO3L; DR3LG; MGC129581; MGC20669; secreted form; TNF-related weak inducer of apoptosis; TNF12_HUMAN; TNFSF 12; Tnfsf12; TNFSF12 protein; Tumor necrosis factor (ligand) superfamily member 12; Tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; anti-TNFSF12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TNFSF12 antibody detects endogenous levels of total TNFSF12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
249
Applicable Applications for anti-TNFSF12 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TNFSF12
Subcellular Location
Secreted and Cell Membrane.
Tissue Specificity
Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis on HuvEc, Jurkat and 293 cell lysate using TNFSF12 Antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on HuvEc, Jurkat and 293 cell lysate using TNFSF12 Antibody.The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry)
(AAA320965 at 1/100 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA320965 at 1/100 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA320965 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA320965 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-TNFSF12 antibody
Description: TNFSF12 Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Belongs to the tumor necrosis factor family. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q.
Subunit Structure: Homotrimer (Probable). Interacts with the angiogenic factor AGGF1/VG5Q.
Post-translational Modifications: The soluble form derives from the membrane form by proteolytic processing.
Similarity: Belongs to the tumor necrosis factor family.
Subunit Structure: Homotrimer (Probable). Interacts with the angiogenic factor AGGF1/VG5Q.
Post-translational Modifications: The soluble form derives from the membrane form by proteolytic processing.
Similarity: Belongs to the tumor necrosis factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kDa
Predicted: 28 kDa
Predicted: 28 kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 12 proprotein
NCBI Official Synonym Full Names
TNF superfamily member 12
NCBI Official Symbol
TNFSF12
NCBI Official Synonym Symbols
APO3L; DR3LG; TWEAK; TNLG4A
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
UniProt Gene Name
TNFSF12
UniProt Synonym Gene Names
APO3L; DR3LG; TWEAK
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Product Notes
The TNFSF12 tnfsf12 (Catalog #AAA320965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFSF12 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNFSF12 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TNFSF12 tnfsf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFSF12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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