IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA234453 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human, Rat TNIP1 Polyclonal Antibody | anti-TNIP1 antibody
TNIP1 Antibody
Gene Names
TNIP1; VAN; NAF1; ABIN-1; nip40-1
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
TNIP1, Antibody; TNIP1 Antibody; TNFAIP3-interacting protein 1; A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; Nef-associated factor 1; Naf1; Nip40-1; Virion-associated nuclear shuttling protein; VAN; hVAN; TNIP1; KIAA0113; NAF1; anti-TNIP1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
619
Applicable Applications for anti-TNIP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human TNFAIP3-interacting protein 1 protein (526-636AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA234453 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohistochemisry)
(Immunofluorescence staining of Hela cells with AAA234453 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IHC (Immunohiostchemistry)
(IHC image of AAA234453 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system.)
IHC (Immunohiostchemistry)
(IHC image of AAA234453 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system.)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat kidney tissueAll lanes: TNIP1 antibody at 7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 72, 67, 65, 63, 62 KDaObserved band size: 72 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Rat kidney tissueAll lanes: TNIP1 antibody at 7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 72, 67, 65, 63, 62 KDaObserved band size: 72 KDa)
Related Product Information for anti-TNIP1 antibody
Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
Product Categories/Family for anti-TNIP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,103 Da
NCBI Official Full Name
TNFAIP3-interacting protein 1 isoform 1
NCBI Official Synonym Full Names
TNFAIP3 interacting protein 1
NCBI Official Symbol
TNIP1
NCBI Official Synonym Symbols
VAN; NAF1; ABIN-1; nip40-1
NCBI Protein Information
TNFAIP3-interacting protein 1
UniProt Protein Name
TNFAIP3-interacting protein 1
UniProt Gene Name
TNIP1
UniProt Synonym Gene Names
KIAA0113; NAF1; ABIN-1; Naf1; VAN; hVAN
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Product Notes
The TNIP1 tnip1 (Catalog #AAA234453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNIP1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNIP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TNIP1 tnip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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