Rabbit TOB1 Polyclonal Antibody | anti-TOB1 antibody

TOB1 (Phospho-Ser164) Antibody

Cat. Num. AAA304312

• Gene Names: TOB1; TOB; TROB; APRO6; PIG49; TROB1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: TOB1, Antibody; TOB1 (Phospho-Ser164) Antibody; TOB; Transducer of erbB-2 1; anti-TOB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 345

• Applicable Applications for anti-TOB1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.
• Modification: Phospho-Ser164
• Target Name: TOB1
• Preparation and Storage: Store at -20 degree C/1 year

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot)

(Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.)

Related Product Information for anti-TOB1 antibody

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.Matsuda S., Oncogene 12:705-713(1996).Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

Product Categories/Family for anti-TOB1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 345197216
• NCBI GeneID: 10140
• NCBI Accession #: NP_001230806.1
• NCBI GenBank Nucleotide #: NM_001243877.1
• UniProt Accession #: P50616; Q4KMQ0; B2R9T0; D3DTY3
• Molecular Weight: 38,155 Da
• NCBI Official Full Name: protein Tob1 isoform 1
• NCBI Official Synonym Full Names: transducer of ERBB2, 1
• NCBI Official Symbol: TOB1
• NCBI Official Synonym Symbols: TOB; TROB; APRO6; PIG49; TROB1
• NCBI Protein Information: protein Tob1
• UniProt Protein Name: Protein Tob1
• UniProt Gene Name: TOB1
• UniProt Synonym Gene Names: TOB; TROB1
• UniProt Entry Name: TOB1_HUMAN