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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
Rabbit Toll Like Receptor 4 (TLR4) Polyclonal Antibody | anti-TLR4 antibody
Polyclonal Antibody to Toll Like Receptor 4 (TLR4)
Gene Names
Tlr4; Lps; Ly87; Ran/M1; Rasl2-8
Reactivity
Mouse, Human, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Toll Like Receptor 4 (TLR4), Antibody; Polyclonal Antibody to Toll Like Receptor 4 (TLR4); anti-TLR4 antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TLR4. It has been selected for its ability to recognize TLR4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- NPCIE VVPNITYQCM DQKLSKVPDD IPSSTKNIDL SFNPLKILKS YSFSNFSELQ WLDLSRCEIE TIEDKAWHGL HHLSNLILTG NPIQSFSPGS FSGLTSLENL VAVETKLASL ESFPIGQLIT LKKLNVAHNF IHSCKLPAYF SNLTNLVHVD LSYNYIQTIT VNDLQFLREN PQVNLSLDMS LNPIDFIQDQ AFQGIKLHEL TLRGNFNSSN IMKTCLQNLA GLHVHRLILG EFKDERNLE
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- NPCIE VVPNITYQCM DQKLSKVPDD IPSSTKNIDL SFNPLKILKS YSFSNFSELQ WLDLSRCEIE TIEDKAWHGL HHLSNLILTG NPIQSFSPGS FSGLTSLENL VAVETKLASL ESFPIGQLIT LKKLNVAHNF IHSCKLPAYF SNLTNLVHVD LSYNYIQTIT VNDLQFLREN PQVNLSLDMS LNPIDFIQDQ AFQGIKLHEL TLRGNFNSSN IMKTCLQNLA GLHVHRLILG EFKDERNLE
Sequence Length
839
Applicable Applications for anti-TLR4 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant TLR4 (Asn26~Glu269) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
IHC (Immunohistochemisry)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
IHC (Immunohistochemisry)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
IHC (Immunohiostchemistry)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue)
IHC (Immunohiostchemistry)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue)
WB (Western Blot)
(Western Blot: Sample: Recombinant TLR4, Mouse.)
WB (Western Blot)
(Western Blot: Sample: Recombinant TLR4, Mouse.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,519 Da
NCBI Official Full Name
toll-like receptor 4
NCBI Official Synonym Full Names
toll-like receptor 4
NCBI Official Symbol
Tlr4
NCBI Official Synonym Symbols
Lps; Ly87; Ran/M1; Rasl2-8
NCBI Protein Information
toll-like receptor 4
UniProt Protein Name
Toll-like receptor 4
UniProt Gene Name
Tlr4
UniProt Synonym Gene Names
Lps
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Product Notes
The TLR4 tlr4 (Catalog #AAA132547) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Toll Like Receptor 4 (TLR4) reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Toll Like Receptor 4 (TLR4) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), ELISA, IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TLR4 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- NPCIE VVPNITYQCM DQKLSKVPDD IPSSTKNIDL SFNPLKILKS YSFSNFSELQ WLDLSRCEIE TIEDKAWHGL HHLSNLILTG NPIQSFSPGS FSGLTSLENL VAVETKLASL ESFPIGQLIT LKKLNVAHNF IHSCKLPAYF SNLTNLVHVD LSYNYIQTIT VNDLQFLREN PQVNLSLDMS LNPIDFIQDQ AFQGIKLHEL TLRGNFNSSN IMKTCLQNLA GLHVHRLILG EFKDERNLE. It is sometimes possible for the material contained within the vial of "Toll Like Receptor 4 (TLR4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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